Agrobacterium Mediated Transformation of OsTZF8 Gene in Oryza sativa for Drought Stress Tolerance | [Sarhad Journal of Agriculture • 2020]

Author(s):

1. Muhammad Medrar Hussain: Institute of Biotechnology and Genetic Engineering, The University of Agriculture,Peshawar, Khyber Pakhtunkhwa, Postal Code 25130,Pakistan

2. Asad Jan: Institute of Biotechnology and Genetic Engineering, The University of Agriculture,Peshawar, Khyber Pakhtunkhwa, Postal Code 25130,Pakistan

3. Sayyar Khan Kazi: Institute of Biotechnology and Genetic Engineering, The University of Agriculture,Peshawar, Khyber Pakhtunkhwa, Postal Code 25130,Pakistan

Abstract:

| Rice (Oryza sativa L.) is a commonly consumed crop nourishing more than half of the human population. different environmental stresses greatly afect cash crops. Among these, drought is a serious abiotic stress efecting rice productivity. Therefore, the current study was designed to know the role of OsTZF8 gene in conferring tolerance to drought stress. For this purpose, OsTZF8 gene was over-expressed in Oryza sativa subspecies indica variety Swat-1. Through Agrobacterium mediated transformation, OsTZF8 gene (pBIG-Ubi-OsTZF8) was successfully transferred to rice calli. From these rice calli transgenic plants were regenerated and established in greenhouse. For the evaluation of OsTZF8 gene role in drought stress tolerance, two weeks old rice seedling were subjected to drought stress. OsTZF8-OX transgenic indica line A and B displayed 69% and 64% survival rates respectively compared to 33% of control. These results confirmed that due to overexpression of OsTZF8 gene, transgenic line A and B displayed considerable level of drought stress tolerance. Additionally, the chlorophyll content of top four leaves of OsTZF8-OX transgenic indica line A and B were observed to be high compared to control. In conclusion our study revealed that over-expression of OsTZF8 gene has significant role in conferring tolerance to drought stress in rice.

Page(s): 702-713

DOI: DOI not available

Published: Journal: Sarhad Journal of Agriculture, Volume: 36, Issue: 2, Year: 2020

Keywords:

PCR , chlorophyll , drought stress , transformation , OsTZF8

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