The lectin content of cotyledonary callus from Canavalia brasiliensis (Mart. ex. Benth). | [Asian Journal of Plant Sciences • 2005]

Author(s):

1. Fabia M. B. Da Silva: Laboratorio de Lectinas e Glicoconjugados, Departamento Bioquimica e Biologia Molecular, Universidade Federal do Ceara, Caixa Postal 6020, CEP 60455-900 Fortaleza-CE, Brazil

2. Renato A. Moreira: Laboratorio de Lectinas e Glicoconjugados, Departamento Bioquimica e Biologia Molecular, Universidade Federal do Ceara, Caixa Postal 6020, CEP 60455-900 Fortaleza-CE, Brazil

3. Ana C. G. Horta: Laboratorio de Lectinas e Glicoconjugados, Departamento Bioquimica e Biologia Molecular, Universidade Federal do Ceara, Caixa Postal 6020, CEP 60455-900 Fortaleza-CE, Brazil

4. Andre Luis C. Silva: Grupo de Biofisica Molecular e Espectroscopia Instituto de Fisica de Sao Carlos, Universidade de Sao Paulo, Caixa Postal 369, CEP, 13560-970, Sao Carlos, SP, Brazil

Abstract:

Callus cultures established from cotyledons of mature seeds were used to investigate the lectin biosynthesis in Canavalia brasiliensis (Mart, ex. Benth). The higher percentage of callus formation were found in MS medium containing 2, 4-D, kinetin and sucrose over 30 days. The callus growth curves showed a sigmoidal pattern with five distinct phases. After 28 days, the calli were freeze dried, macerated and submitted to extraction with buffers of different pH values and used for determination of soluble protein content. The lectin (ConBr) and other protein were identified by SDS-PAGE and immunospecific tests. Some differences were found between soluble protein content and bands (intensity and number) for callus during the growth curve. The lectin was detected in the callus until the third subculture.

Page(s): 214-219

DOI: DOI not available

Published: Journal: Asian Journal of Plant Sciences, Volume: 4, Issue: 3, Year: 2005

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