A Comparative study of- Axillary shoot proliferation from the Nodal Explants of three varities Pummelo (Citrus gradis (L-)Osb | [Biotechnology • 2004]

Author(s):

1. F. Begum: Department of Botany, University of Rajshahi, Rajshahi¬6205, Bangladesh

2. M. N. Amin: Plant Tissue Culture Section, BCSIR Laboratories, Dhaka, Bangladesh

3. S. Islam: Plant Tissue Culture Section, BCSIR Laboratories, Dhaka, Bangladesh

4. M. A. K. Azad: Department of Forest Science, Utsunomiya University, Japan

Abstract:

Nodal segments of in vitro germinated seedlings of three pummelo varieties (Var-1 (pulp is pink colour),Var-2 (pulp is white colour) and Var-3 (pulp is. red colour) were cultured on half-strength Ms medium for axillary shoot proliferation. A large number of shoot buds were produced when such four weeks old culture were subcultured on half-strength MS medium containing 1.0 mg 1". BAP Roots were induced when the isolated individual shoots were cultured on half-strength containing 0.1 mg I" each of NAA, IBA or IAA. Cent percent root were observed on half-strength MS medium having 0.1 mg 1"` NAA. These in vitro grown plantlets were then successfully transferred to outside natural condition through successive phases of acclimatization. About 93% of the regenerated plantlets survived under ex vitro condition.

Page(s): 56-62

DOI: DOI not available

Published: Journal: Biotechnology, Volume: 3, Issue: 1, Year: 2004

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