Abstract:
Vector-borne diseases (VBDs) remain a significant public health and economic challenge in Pakistan, affecting both human and animal populations. Tick-borne pathogens, including Anaplasma, Babesia, and Theileria species, pose threats to livestock health, food security, and zoonotic safety. Rapid, accurate, and fielddeployable surveillance is essential for early detection and control. The present study demonstrates the application of Nanopore sequencing as a core tool in an integrated surveillance framework for VBDs, with emphasis on tick-borne infections. Field sampling was conducted in farmed and wildlife animals, followed by portable Nanopore sequencing for real- time pathogen identification. Results show that Nanopore sequencing offers rapid turnaround, high portability, and adaptability in resource- limited settings, making it suitable for on-site diagnostics and outbreak investigations. Despite challenges such as higher raw read error rates and the need for bioinformatics expertise, advancements in sequencing chemistry, base calling algorithms, and analysis workflows are improving accuracy and usability. This approach strengthens One Health surveillance networks, supports timely interventions, and reduces diagnostic delays for both veterinary and public health sectors. Integrating Nanopore sequencing with community-based vector monitoring and GIS tracking can significantly enhance Pakistan’s preparedness against tick-borne and other vector-borne diseases, contributing to improved animal health, food security, and zoonotic risk mitigation.
Page(s):
46-46
DOI:
DOI not available
Published:
Journal: 4th International Conference of Sciences “Revamped Scientific Outlook of 21st Century, 2025” , November 12,2025, Volume: 1, Issue: 1, Year: 2025
Keywords:
Tickborne pathogens
,
Nanopore sequencing
,
Vector borne diseases
,
One Health surveillance