A molecular study of genetic diversity in Shisham (Dalbergia sissoo) plantation of NWFP, Pakistan. | [Pakistan Journal of Botany • 2010]

Author(s):

1. Muhammad Ashraf: NUST Center of Virology and Immunology, National University of Science &Technology, Islamabad, Pakistan

2. Sobia Tabassum: NUST Center of Virology and Immunology, National University of Science &Technology, Islamabad, Pakistan

3. Abdul Samad Mumtaz: Department of Plant Sciences, Quaid-i-Azam University, Islamabad, Pakistan

4. Rabia Riasat: Department of Plant Sciences, Quaid-i-Azam University, Islamabad, Pakistan

Abstract:

Genetic diversity of 22 accessions of Dalbergia sissoo that were collected from the canal, road; farmer’s field and forest sites of N.W.F.P, Pakistan has been studied, by using a finger printing technique “RAPD” (Random Amplified Polymorphism DNA). Out of 20 primers OPA-2 was the primer that allows distinguishing the diseased and healthy accessions. The selected primer was used for identification and for establishing a profiling system to estimate genetic relationships and to evaluate the genetic variability among the accessions. A total of 126 DNA bands or fragments were amplified by using the primers. The UPGMA cluster analysis revealed 2 main clusters among 22 accessions of Dalbergia sissoo based on coefficient of similarity and dissimilarity. Overall 72% similarity and 98% dissimilarity were observed. Low level of genetic variation and high level of genetic relatedness occurred among the canal, road, farmer’s field and forest sites. The accessions were closely related with each other and showed mix pattern of genetic diversity. Thus RAPD markers have the potential to characterize and establish genetic relationships among the accessions of Dalbergia sissoo.

Page(s): 79-88

DOI: DOI not available

Published: Journal: Pakistan Journal of Botany, Volume: 42, Issue: 1, Year: 2010

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