Tetramethylpyrazine Effects on the Expression of Scar-related Genes in Rabbit Benign Biliary Stricture Fibroblasts. | [Journal of College of Physicians and Surgeons--Pakistan : JCPSP • 2016]

Author(s):

1. KeYue Li: Graduate School of Tianjin Medical University, 300070, Tianjin, China

2. Cheng Xian Shi: Department of Hepatobiliary Surgery, Guizhou Provincial People's Hospital, Guiyang 550002, Guizhou Province, China

3. Jian Zhao Huang: Department of Hepatobiliary Surgery, Guizhou Provincial People's Hospital, Guiyang 550002, Guizhou Province, China

4. Keli Tang: Department of Hepatobiliary Surgery, Guizhou Provincial People's Hospital, Guiyang 550002, Guizhou Province, China

Abstract:

Objective: To investigate the effects of tetramethylpyrazine (TMP) on transforming growth factor-ß1 (TGF- ß1), a-smooth muscle actin (a-SMA), and neuronal regeneration related protein (P311) in benign biliary stricture fibroblasts of rabbit. Study Design: An experimental study. Place and Duration of Study: Guizhou Medical University, Guiyang, Guizhou, China, from April to December 2015. Methodology: Fibroblasts isolated from rabbits following benign biliary stricture were cultured and treated with different concentrations of TMP (0.08, 0.4, and 2.0 mg/ml). TMP-treated cells and non-treated control groups were incubated for 48-hours, and proliferation was assessed using the cell counting kit-8 assay. The mRNA expressions of TGF-ß1, a-SMA, and P311 were assessed by quantitative RT-PCR. Protein expressions of TGF-ß1 and a-SMA were investigated by Western blotting. Results: Treatment with TMP significantly reduced the proliferation of benign biliary stricture fibroblasts, and significantly attenuated both the mRNA and protein expressions of TGF-ß1, a-SMA, and P311 (p < 0.05) in a dose-dependent manner. Conclusion: TMP significantly reduced the proliferation of benign biliary stricture fibroblasts, and significantly downregulated the mRNA/protein expression of TGF-ß1, a-SMA, and P311. Therefore, TMP may be a therapeutic option for the prevention of benign biliary stricture.

Page(s): 813-817

DOI: DOI not available

Published: Journal: Journal of College of Physicians and Surgeons--Pakistan : JCPSP, Volume: 26, Issue: 10, Year: 2016

Keywords:

Keywords are not available for this article.

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