Toxicity of ciprofloxacin; preventive role of zinc chloride on appearance of secondary ossification centers in wistar albino rat litters. | [Professional Medical Journal • 2004]

Author(s):

1. Muhammad Aslam Channa: Department of Anatomy, Jinnah Postgraduate Medical Center, Karachi, Pakistan

2. Muhammad Ashfaque: Department of Anatomy, Jinnah Postgraduate Medical Center, Karachi, Pakistan

3. Sibghatullah Sanghi: Department of Anatomy, Jinnah Postgraduate Medical Center, Karachi, Pakistan

4. Muhammad Baqir Soomro: Department of Anatomy, Jinnah Postgraduate Medical Center, Karachi, Pakistan

Abstract:

Objectives: To evaluate the preventive role of Zinc Chloride on Toxicity of Ciprofloxacin administration on secondary ossification centers in wistar albino rat litters. Design of Study: Prospective and comparative animal study carried out on experimental Wistar Albino Rats. Setting: The study was carried out in the department of Anatomy Basic Medical Sciences Institute, Jinnah Postgraduate Medical Center JPMC) Karachi from 2001 to 2002. Methods: Ciprofloxacin and Zinc Chloride were administered to newly born albino rat pups separately and simultaneously at a dose of 20 mg/kg body weight and 120 ug/100g body weight respectively mtraperitoneally twice daily from day 1 to 14 after birth. These animals were killed by deep either anaesthesia and fixed in 80% alcohol. They were then bulk stained with alizarin Red S and alcian blue. Finally they were cleared in 4% KOH and stored in Glycerin. The fore and hind limbs were disarticulated from the axial skeleton and observed under stereo-microscope for evidence of skeletal differentiation in the form of presence of secondary ossification centers in ling bones. The time of appearance of these centers were noted and compared statistically with those in the control animals. Results: This study revealed that the secondary ossification centers in long bones were delayed in experimental animals and simultaneous treatment with Zinc Chloride partially prevented toxicity of ciprofloxacin as compared with controls. Conclusion: these results strongly suggest that ciprofloxacin causes a risk to skeletal differentiation and therefore to its growth. However the ciprofloxacin toxicity could be partially prevented by simultaneous administration of Zinc Chloride in wistar albino rat litters.

Page(s): 72-78

DOI: DOI not available

Published: Journal: Professional Medical Journal, Volume: 11, Issue: 1, Year: 2004

Keywords:

Keywords are not available for this article.

References:

References are not available for this document.

Citations

Citations are not available for this document.

Citations

Downloads

Views