Abstract:
Lipoxygenase (LOX, EC 1.13.11.12) constitutes the heterogenous family of lipid per-oxidizing enzyme which catalyzes the oxidation of poly-unsaturated fatty acids (PUFA) particularly linoleic acid, linolenic acid and arachidonic acid into lipid hydroperoxides known as hydroxy octadecadienoic acid. LOX enzyme is widely distributed in a large variety of organisms such as mammals, plants, fungi, algae and bacteria and performs numerous biological functions such as cellular signaling, inflammation, pest resistance, senescence development, wound healing, and mycotoxin production. In animals, lipoxygenase (LOX) metabolizes arachidonic acid into eicosanoids, a lipid-signaling molecules. The current study evaluated the extraction and partial purification of lipoxygenase (LOX) from Capra aegagrus hircus (Goat) tissues as well as biochemical characterization of LOX extracted from goat tissues. The extraction of LOX enzyme from goat tissues (heart, liver and brain) was carried out through homogenization using phosphate buffer at pH-9, followed by precipitation with 30% and 70% ammonium sulphate. Purification of LOX in heart, liver and brain was (7.34, 7.537 and 10.558) folds in (57.98, 67.14, and 78.16)% yield with specific activity of (1.101, 2.967, and 3.098) U/mg, respectively. The optimum pH value for LOX extracted from both liver and brain was determined as pH 5.2, while pH 5.6 was the optimum value for LOX activity for heart. The LOX obtained from heart and brain tissues showed maximum activity at 40°C. In contrast, the purified LOX from liver tissues exhibited the highest activity at 70°C. The initial findings obtained from biochemical characterization of LOX in different tissues of Capra aegagrus hircus (goat) will contribute to the advance research on said enzyme and its mechanism of action in goats.
Page(s):
125-125
DOI:
DOI not available
Published:
Journal: Abstract Book on International Conference on Food and Applied Sciences (ICFAS-23) 3-5 August 23, Volume: 0, Issue: 0, Year: 2023
Keywords:
lipoxygenase
,
kinetic parameters
,
Capra aegagrus hircus
,
Ammonium Sulphate
,
Linoleic Acid