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The effect of myo-inositol on the growth of callus tissue in Gossypium hirsutum L.
Author(s):
1. Lal Hussain Akhtar: University of Wales, Bangor, Gwynedd, UK
2. John Gorham: University of Wales, Bangor, Gwynedd, UK
3. M. Anwar Mirza: Central Cotton Research Institute, Multan, Pakistan
Abstract:
Callus was induced from leaf explants of Gossypium hirsutum L cv. Acala SJ2 on medium containing LS basal salts and vitamin with 2, 4-dichlorophenoxyacetic acid (2, 4-D), benzylaminopurin (BAP) and gibberellic acid (GA3). Five different concentrations of myo-inositol were tested for the optimum growth of callus tissues in vitro. The callus cells required myo-inositol and showed increasing growth upto an optimum of 100 mg 1 1 . Further increase of the myo-inositol concentration resulted in decreasing growth. Growth promotion may be due to a high cell division rate and not to cell enlargement. It means that the callus growth of Acala SJ2 is dependent upon myo-inositol.
Page(s): 91-98
DOI: DOI not available
Published: Journal: Pakistan Journal of Plant Sciences, Volume: 1, Issue: 1, Year: 1995
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