Therapeutic potential of Dicliptera roxburghiana against CCl4 I induced renal toxicity in mice | [Abstract Book on International Conference on Food and Applied Sciences (ICFAS-23) 3-5 August 23 • 2023]

Author(s):

1. Bushra Ahmad: Department of Biochemistry, Shaheed Benazir Bhutto Women University, Peshawar, Pakistan

2. M. Rashid Khan: Department of Biochemistry, Faculty of Biological Sciences, QAU, Islamabad, Pakistan

3. A. Jamil: Department of Plant Breeding and Genetics, University of Agriculture, D. I Khan

4. Ayesha Siddique: 1Department of Biochemistry, Shaheed Benazir Bhutto Women University, Peshawar, Pakistan

5. Umama Syed: Department of Biochemistry, Shaheed Benazir Bhutto Women University, Peshawar, Pakistan

Abstract:

Plants are efficient source of natural antioxidant against free radicals causing kidney damages. Dicliptera roxburghiana possesses phytochemicals having potent antioxidant activity that may provide an effective alternate therapy against free radicals causing nephrotoxicity. This study was designed to investigate the effect of methanol extract of D. roxburghiana (DRME) in CCl4 induced renal injuries in mice. 42 male Balb c mice were treated with alternatively managed doses for 30 days. Group I was considered as control whereas group II was injected i.p. with DMSO (1 ml/kg b.w.) and olive oil (1 ml/kg b.w.). Group III was administered with only CCl4 (1 ml/kg b.w; 20% in olive oil). Group IV was injected with CCl4 and silymarin (50 mg/kg b.w.). Group V and VI were treated with CCl4 along with injected DRME (40; 60 mg/kg b.w.) Group VII was treated alone with DRME (60 mg/kg b.w.). Serum proteins and urine profile were investigated. Activities of tissue enzyme like catalase, peroxidase, superoxide dismutase and glutathione-S-transferase, Glutathione reductase, GSH and ?-GT were evaluated. Histopathalogical observations, total protein contents, lipid peroxidation, DNA damage and relative weight were also analyzed. DRME, dose dependently, prevented the intoxications in renal tissue. CCl4 increases the organ weight and decreases the body weight, both these drastic changes were reversed to the normal with DRME treatment. Abnormal urine profile was noticed in CCl4 treated mice. Serum proteins and evels of catalase, peroxidase, superoxide dismutase, glutathione-S-transferase and glutathione were reduced in kidney homogenates of CCl4 treated group. CCl4 also causes abnormalities in biomolecular profile of kidney tissue , all these abnormalities were overcome with the DRME treatments. These results are an evidence of the renalprotective role of DRMEagainst CCl4 induced nephrotoxicity in mice which may be due to its antioxidant compounds.

Page(s): 318-318

DOI: DOI not available

Published: Journal: Abstract Book on International Conference on Food and Applied Sciences (ICFAS-23) 3-5 August 23, Volume: 0, Issue: 0, Year: 2023

Keywords:

antioxidant enzymes , CCl4 , Kidney , lipid peroxidation , Dicliptera roxburghiana

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