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Determination of reactive oxygen species in Japanese quails at different age and genders
Author(s):
1. Rabia Bashir: Department of Zoology, Wildlife & Fisheries, University of Agriculture Faisalabad, Pakistan
2. Rehmatullah Shahid: Department of Zoology, Wildlife & Fisheries, University of Agriculture Faisalabad, Pakistan
3. Razia Kausar: Department of Zoology, Wildlife & Fisheries, University of Agriculture Faisalabad, Pakistan
4. Hasooba Hira: Department of Zoology, Wildlife & Fisheries, University of Agriculture Faisalabad, Pakistan
5. Tayyba Batool: Department of Zoology, Wildlife & Fisheries, University of Agriculture Faisalabad, Pakistan
Abstract:
The Japanese quails have rapid growth, high rate of egg production, short generation interval and early sexual maturity. They require a small place to grow, short incubation period, less susceptibility to common diseases, less and low cost feed requirements. Reactive oxygen species are free radicals that are derived from molecular oxygen. Those molecules are produced as byproduct in the process of aerobic respiration. The present study was done on 30 Japanese Quails which were divided into 5 random groups i.e. young Japanese quails (n=6), adult males (n=6), adult females (n=6), old males (n=6) and old females (n=6). Blood samples were drawn by slaughtering the birds and the blood samples were centrifuged at 4000 g for 10 minutes. Plasma was separated and stored in appendorf at -20 ?C to ascertain different values of reactive oxygen species like total oxidant status (TOS), total antioxidant capacity (TAC), glutathione reductase, glutathione oxidase, sodium oxide dismutase, paraoxonase, arylesterase, Serum glutamate pyruvate transaminase, malondialdehyde, catalase, vitamin A, C and D by using spectrophotometer at a specific wavelength according to the tested parameters. It was observed that the values of these parameters were significantly different among the different gender and age groups of Japanese quails. The whole blood samples were analyzed by automated hematological system for complete blood cells (CBC) count. The result revealed that the mean values of TOS, TAC, glutathione reductase, paraoxonase, catalase, MDA, vitamin A, vitamin C and vitamin E were significantly different from each other. All the values of red blood cells and packed cell volume were non-significant.
Page(s): 119-119
DOI: DOI not available
Published: Journal: Abstract Book on International Conference on Food and Applied Sciences (ICFAS-23) 3-5 August 23, Volume: 0, Issue: 0, Year: 2023
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