Alkaloid and phenolic compounds of Xylopia aromatica inhibits tumor growth by down-regulating matrix metalloproteinase-2 (MMP-2) expression | [Pakistan Journal of Pharmaceutical Sciences • 2021]

Author(s):

1. Izabela Natalia Faria Gomes: Experimental Pathology Laboratory - Federal University of Sao Joao del Rei - Centro-Oeste D. Lindu Campus (UFSJ/CCO) -400, Sebastiao Goncalves Coelho,Chanadour, Divinopolis MG,Brazil

2. Ana Gabriela Silva: Experimental Pathology Laboratory - Federal University of Sao Joao del Rei - Centro-Oeste D. Lindu Campus (UFSJ/CCO) -400, Sebastiao Goncalves Coelho,Chanadour, Divinopolis MG,Brazil

3. Gustavo Fernando de Frazao Lima: Experimental Pathology Laboratory - Federal University of Sao Joao del Rei - Centro-Oeste D. Lindu Campus (UFSJ/CCO) -400, Sebastiao Goncalves Coelho,Chanadour, Divinopolis MG,Brazil

4. Tamara Ribeiro Longatti: Experimental Pathology Laboratory - Federal University of Sao Joao del Rei - Centro-Oeste D. Lindu Campus (UFSJ/CCO) -400, Sebastiao Goncalves Coelho,Chanadour, Divinopolis MG,Brazil

5. Lucas Fernandes Do Carmo: Laboratory of Organic Synthesis and Nano Structures, Federal University of Sao Joao del Rei - Centro-Oeste D. Lindu Campus (UFSJ/CCO) -400, Sebastiao Goncalves Coelho,Chanadour, Divinopolis MG,Brazil

6. Jose Augusto Ferreira Perez Villar: Laboratory of Organic Synthesis and Nano Structures, Federal University of Sao Joao del Rei - Centro-Oeste D. Lindu Campus (UFSJ/CCO) -400, Sebastiao Goncalves Coelho,Chanadour, Divinopolis MG,Brazil

7. Arali Aparecida Da Costa Araujo: University of the State of Minas Gerais (UEMG), Rua Vereador Geraldo Moises da Silva s/no - Campus Universitario,Setor Universitario, Ituiutaba, MG,Brazil

8. Ralph Gruppi Tome: Tissue Processing Laboratory, Federal University of Sao Joao del Rei - Centro-Oeste D. Lindu Campus (UFSJ/CCO) -400, Sebastiao Gonçalves Coelho,Chanadour, Divinopolis MG,Brazil

9. Helio Batista Dos Santos: Tissue Processing Laboratory, Federal University of Sao Joao del Rei - Centro-Oeste D. Lindu Campus (UFSJ/CCO) -400, Sebastiao Gonçalves Coelho,Chanadour, Divinopolis MG,Brazil

10. Rosy Iara Maciel De Azambuja Ribeiro: Experimental Pathology Laboratory - Federal University of Sao Joao del Rei - Centro-Oeste D. Lindu Campus (UFSJ/CCO) -400, Sebastiao Goncalves Coelho,Chanadour, Divinopolis MG,Brazil

Abstract:

Annonacea species have been reported to possess antitumor properties. However, the in vitro and in vivo antitumor activities of Xylopia aromatica (Annonacea) have not yet been elucidated. This study aimed to investigate the effects of Xylopia aromatica leaves hexane fraction (XaHF) on Ehrlich ascites carcinoma cells lines (EAC), both in vitro and in vivo. In vitro assays revealed a significant cytotoxic effect with the two lower XaHF concentrations (62.5 and 32.3mg/mL). EAC (2.5x106 cells) were inoculated in the right flank of Swiss mice, and the animals were treated intraperitoneally with 32.3mg kg-1 of XaHF daily, for 20 days. Our findings indicate that XaHF suppressed the growth of EAC in vivo, with a significant decrease (46%) in tumor volume. There was also a decrease in the necrosis area (71%), inflammatory infiltrate, and MMP-2 expression. High-Performance Liquid Chromatography with Diode Array Detector (HPLC- DAD) identified secondary metabolites possibly related to phenolic acids, flavonoids, and alkaloids. Thus, the results confirmed the antitumoral activity that may be related to the presence of the identified metabolites in XaHF extract

Page(s): 599-606

DOI: DOI not available

Published: Journal: Pakistan Journal of Pharmaceutical Sciences, Volume: 34, Issue: 2, Year: 2021

Keywords:

Medicinal Plants , Secondary metabolites , matrix metalloproteinase , Ehrlich tumor , antitumoral activity

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