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Transcriptome Analysis Reveals That Blocking the Ethylene Signal Transduction Pathway is a Key Point for 2,4-D Inhibited Shikonin Biosynthesis in Lithospermum erythrorhizon (Boraginaceae).
Author(s):
1. YONGHUI LIAO: Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University,Nanjing 210037,P. R. China;Institute of Plant Molecular Biology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University,Nanjing 210093,P.R. China
2. CHENGYI TANG: Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University,Nanjing 210037,P. R. China;Institute of Plant Molecular Biology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University,Nanjing 210093,P.R. China
3. RONGJUN FANG: Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University,Nanjing 210037,P. R. China;Institute of Plant Molecular Biology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University,Nanjing 210093,P.R. China
4. YU ZHU: Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University,Nanjing 210037,P. R. China;Institute of Plant Molecular Biology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University,Nanjing 210093,P.R. China
5. FENGYAO WU: Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University,Nanjing 210037,P. R. China;Institute of Plant Molecular Biology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University,Nanjing 210093,P.R. China
6. PINGZHI ZHAO: Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University,Nanjing 210037,P. R. China;Institute of Plant Molecular Biology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University,Nanjing 210093,P.R. China
7. SHOUCHENG HUANG: College of Life Science, Anhui Science and Technology University,Fengyang 233100,P.R. China;Institute of Plant Molecular Biology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University,Nanjing 210093,P.R. China
8. XIAOMING WANG: Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University,Nanjing 210037,P. R. China;Institute of Plant Molecular Biology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University,Nanjing 210093,P.R. China
9. YANJUN PANG: Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University,Nanjing 210037,P. R. China;Institute of Plant Molecular Biology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University,Nanjing 210093,P.R. China
10. RONGWU YANG: Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University,Nanjing 210037,P. R. China;Institute of Plant Molecular Biology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University,Nanjing 210093,P.R. China
11. GUIHUA LU: Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University,Nanjing 210037,P. R. ChinaInstitute of Plant Molecular Biology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University,Nanjing 210093,P.R. China
12. JINLIANG QI: Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University,Nanjing 210037,P. R. ChinaInstitute of Plant Molecular Biology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University,Nanjing 210093,P.R. China
13. YONGHUA YANG: Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University,Nanjing 210037,P. R. ChinaInstitute of Plant Molecular Biology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University,Nanjing 210093,P.R. China
Abstract:
The synthetic growth hormone2,4-dichlorophenoxyacetic acid (2,4-D) significantly inhibits shikonin and its derivatives biosynthesis in the medicinal plant Lithospermum erythrorhizon. However, the molecular mechanism of this regulation remains unclear.In this study, we attempt to address this issue by comparing the transcriptome of 2,4-D treated cell cultureswith that of the control (CK). A total of 216 up-regulated and 269 down-regulated genes by 2,4-D were discovered. Gene ontology (GO) enrichment analysis revealed that differentially expressed genes (DEGs) were statistically significantly related to the metabolic process. Pathway classification enrichment analysis further confirmed that the DEGs were mainly related to the secondary metabolism. More importantly, 2,4-D has no effect on the two key enzyme genes ACS and ACO for ethylene biosynthesis. However,2,4-D significantly down-regulated the genes directly involved in ethylene signal transduction pathway (ESTP), including HMA5, EIN3, and ORCA3. 2,4-D also down-regulated two indirect genes, LOX and GA2ox, thereby conferring the jasmonate (JA) and GA biosynthesis, which regulates ESTP by targeting EIN3. By blocking ESTP, 2,4-D finally downregulated the phenylpropanoid formation-related genes, thereby conferring shikonin and its derivatives biosynthesis. Our findingsprovide new hints for the deep understanding of the molecular mechanism of shikonin formation.
Page(s): 1451-1458
DOI: DOI not available
Published: Journal: Pakistan Journal of Botany, Volume: 50, Issue: 4, Year: 2018
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