Abstract:
Treatments of alfalfa (Vicia faba L.) seeds with gibberellic acid (GA3), fertilizer, potassium oxide (K2O) and the auxin inhibitor N-meta-tolyl-phathalamic acid (Tomaset) showed a decrease in mitotic index and increased the percentage of the chromosomal abnormalities. The harmful effect was high with the use of tomaset, moderate with K2O and low in plants treated with GA3. SDS-PAGE studies revealed qualitative and quantitative variation in seed protein among the treatments. The number of polypeptide bands varied from 25 bands in control plants to 15 bands in plants treated with 2X tomaset. A different specific polypeptide band was observed as a newly synthesized band with each chemical, a band with (MW) 22.04 kDa and very faint small band with MW 14.5 kDa was induced with the use of K2O, 39.7 kDa band induced in seeds treated with tomaset, while the band 48.9 kDa was observed in control and seeds treated with the higher concentration of GA3 only. The results showed that tomaset was the most harmful chemical used.
Page(s):
499-504
DOI:
DOI not available
Published:
Journal: International Journal of Agriculture and Biology, Volume: 10, Issue: 5, Year: 2008