Whole Stevia leaf powder or Rebaudioside A; Effects of supplementation on obesity induced oxidative stress | [Rawal Medical Journal • 2024]

Author(s):

1. Amna Faruqi: Department of Physiology, NUST School of Health Sciences, Islamabad, Pakistan

2. Sadia Mubarak: Department of Physiology, Islamabad Medical & Dental College, Islamabad, Pakistan

3. Sikander Subuctageen: Department of Ophthalmology, Fauji Foundation Hospital, Rawalpindi, Pakistan

4. Tooba Zafar: Department of Physiology, NUST School of Health Sciences, Islamabad, Pakistan

5. Saima Latif Qureishi: Department of Physiology, Islamabad Medical & Dental College, Islamabad, Pakistan

6. Asma Irfan: Department of Physiology, Islamabad Medical & Dental College, Islamabad, Pakistan

Abstract:

Objective: To determine and compare the effect of diet supplementation with dry, powdered Stevia leaf and its highly purified Rebaudioside A glycoside, on obesity induced oxidative stress in a murine model. Methodology: This experimental study included 120 Sprague Dawley male rats divided in four groups with 30 animals in each group. Normal control (Group 1, n=30), was fed an ordinary diet. Ninety rats were made obese by administration of a high fat diet (HFD) for eight weeks and after confirmation of obesity, using Lee Index, were randomly allocated to group 2 (Obese control), group 3 (obese+Stevia) and group 4 (Obese+Rebaudioside A). HFD was continued for a further six weeks along with supplementation with Stevia leaf powder (group 3) and Rebaudioside A (group 4). Oxidative stress was determined by testing testicular tissue supernatant for Malondialdehyde (MDA) and Catalase (CAT) activity using animal kits. Results: Obesity caused a significant increase in tissue levels of MDA (p<0.001), and activity of Catalase (p><0.001). The administration of Stevia in group 3 showed a significant reduction in tissue MDA levels (p><0.001), while tissue catalase activity indicated a significant increase (p=0.006). Supplementation with Rebaudioside A resulted in a non-significant reduction in tissue MDA level (p=0.055). However, tissue catalase levels were significantly increased (p=0.019). Conclusion: Both preparations possess anti-oxidative activity, however whole stevia leaf powder was found to be more effective than Rebaudioside A in improving oxidative status. >< 0.001), and activity of Catalase (p< 0.001). The administration of Stevia in group 3 showed a significant reduction in tissue MDA levels (p<0.001), while tissue catalase activity indicated a significant increase (p=0.006). Supplementation with Rebaudioside A resulted in a non-significant reduction in tissue MDA level (p=0.055). However, tissue catalase levels were significantly increased (p=0.019). Conclusion: Both preparations possess anti-oxidative activity, however whole stevia leaf powder was found to be more effective than Rebaudioside A in improving oxidative status. >< 0.001), while tissue catalase activity indicated a significant increase (p=0.006). Supplementation with Rebaudioside A resulted in a non-significant reduction in tissue MDA level (p=0.055). However, tissue catalase levels were significantly increased (p=0.019). Conclusion: Both preparations possess anti-oxidative activity, however whole stevia leaf powder was found to be more effective than Rebaudioside A in improving oxidative status.

Page(s): 1-4

DOI: DOI not available

Published: Journal: Rawal Medical Journal, Volume: 49, Issue: 4, Year: 2024

Keywords:

oxidative stress , Stevia , antioxidant , Obesity , Rebaudioside A

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