Evaluation of Genetic Variation among Indian Mustard (Brassica juncea L.) Genotypes by SDS-PAGE Method | [Proceedings of the Pakistan Academy of Sciences: B. Life and Environmental Sciences • 2017]

Author(s):

1. Muhammad Ishaq Ibrahim: Department of Genetics, Hazara University,Mansehra,Pakistan

2. Fida Muhammad Abbasi: Department of Agriculture, Hazara University,Mansehra,Pakistan

3. Malik Ashiq Rabbani: Plant Genetic Resources Institute, National Agricultural Research Centre,Islamabad,Pakistan

4. Inamullah: Department of Genetics, Hazara University,Mansehra,Pakistan

5. Sohail Ahmad Jan: Plant Genetic Resources Institute, National Agricultural Research Centre,Islamabad,Pakistan

6. Muhammad Ilyas: Plant Genetic Resources Institute, National Agricultural Research Centre,Islamabad,Pakistan

7. Haris Khurshid: Department of Agriculture, Hazara University,Mansehra,Pakistan

Abstract:

A comparative study of total seed protein profile was carried out to determine the extent of genetic variability amongst 53 genotypes of Indian mustard [(Brassica juncea L.) germplasm using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) technique. Total seed protein was estimated. To estimate genetic diversity, 12 different types of protein bands were obtained based on the banding pattern of all the genotypes; seven (58%) were polymorphic and five were monomorphic. The protein size base polymorphism revealed the range of protein bands based on their molecular weights which ranged from ~10 kDa to ~180 kDa. Dendrogram was constructed using UPGMA method which divided the genotypes into five groups: group I had 18 genotypes, group II 17 genotypes, group III 3 genotypes, group IV 13 genotypes and group V 2 genotypes. Among the studied genotypes, similarity coefficient values ranged from 17% to 100%. Highest similarity coefficient (i.e., 100%) was recorded between Bj765 and Bj785, Bj790 and Bj791, Bj790 and Bj 794. The observed clusters based on this study exhibited a genetic diversity among these genotypes of Indian mustard on Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) level. Thus, this study revealed that the SDS-PAGE method plays a key role in the study of protein based variation among different genotypes of plant species.

Page(s): 333-339

DOI: DOI not available

Published: Journal: Proceedings of the Pakistan Academy of Sciences: B. Life and Environmental Sciences, Volume: 54, Issue: 4, Year: 2017

Keywords:

genetic diversity , SDSPAGE , cluster analysis , Brassica juncea L

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