A comparative study of chemiluminiscence immunoassay (CLIA) and immunochromatographic technique (ICT) for β-thalassemia (major) patients in local population | [The Journal of Microbiology and Molecular Genetics • 2023]

Author(s):

1. Muhammad Tayyab: Department of Biology, Lahore Garrison University,Sector C, Phase VI, DHA Lahore,Pakistan

2. Saima Irum: Department of Transfusion Medicine & Pathology Azra Naheed Medical College,17-Km, Raiwind Road Lahore, Pakistan

3. Saima Naz: Government Sadiq College Women University,Bahawalpur,Pakistan

4. Muhammad Zaid: Department of Life Sciences, University of Management and Technology,Lahore, Pakistan

5. Adnan Ahmad Khan: Department of Transfusion Medicine, Institute of Blood Transfusion Service Punjab,Lahore, Pakistan

6. Hafiz Zeshan Wadood: Department of Biology, Lahore Garrison University,Sector C, Phase VI, DHA Lahore,Pakistan

Abstract:

The sustainability of beta thalassemia (major) patients is dependent on regular blood transfusion so they are at a greater risk of acquiring transfusion transmitted infections (TTIs). The objective of this research project was to compare the efficiency of two blood screening techniques such as chemiluminescence immunoassay (CLIA) and immunochromatographic technique (ICT) for the detection of transfusion transmitted infections in ß-thalassemia (major) Patients in local population. For this purpose, a total of 250 blood samples were collected and screened through CLIA and ICT. Results showed that 30% of the samples were positive for different infectious diseases like hepatitis B or C virus, Human immunodeficiency virus (HIV) infections, infections with syphilis or malarial agents, while 70% samples were free from any kind of infections. Among all the infectious diseases the incidence of hepatitis C virus (HCV) was the highest i.e., 23.6% and 26.4% incidence of HCV was recorded through ICT and CLIA respectively. The second highest incidence was of hepatitis B virus followed by the other infectious diseases. The results showed that CLIA was more sensitive as compared to ICT in detecting the infections in blood. All blood banks and transfusion centers need to implement authentic screening of blood to counter the TTIs.

Page(s): 15-26

DOI: DOI not available

Published: Journal: The Journal of Microbiology and Molecular Genetics, Volume: 4, Issue: 2, Year: 2023

Keywords:

HCV , blood transfusion , Transfusion Transmitted Infections , Chemiluminescence immunoassay , beta thalassemia major

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