Alkali hydrolyzed conversion of fish scales into protein hydrolysates and evaluation of their antimicrobial activity against different pathogenic bacteria. | [Journal of Chemical Society of Pakistan • 2017]

Author(s):

1. Zahid Hussain: Department of Chemistry, Abdul Wali Khan University, KPK, Pakistan.

2. Nasim Rafiq: Department of Zoology Abdul Wali Khan University, Mardan, Pakistan.

3. Sadaf Niaz: Department of Zoology Abdul Wali Khan University, Mardan, Pakistan.

4. Khalid Mohammed Khan: H. E. J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi, Pakistan.

5. Sobia Dil: H. E. J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi, Pakistan.

6. Zahida Perveen: Department of Biochemistry Abdul Wali Khan University, Mardan, Pakistan.

7. Fazal Mabood: Department of Biological Sciences & Chemistry, College of Arts and Sciences, University of Nizwa, Sultanate of Oman.

8. Farah Jabeen: Department of Chemistry, University of Malakand, KPK, Pakistan.

9. Husan Ara: Department of Botany, Abdul Wali Khan University, KPK, Pakistan.

Abstract:

In this study the fish scales a waste product from the fish industry were converted into water soluble protein hydrolysates on heating with aqueous solution of sodium hydroxide. Those protein hydrolysates were fractionated into different fractions using solvent extraction procedure and were also checked for their antibacterial activity using agar well diffusion method. Their antibacterial activity was also tested against six pathogenic bacteria including Escherichia coli, Margenella morganii, Haemophilus influenzae, Klebsilla pneumoniae, Pseudomonas aeruginosa, and Actenobactor baumannii. All fractions were found active against these bacteria. Their zones of inhibitions were found to vary according to the nature of solvent fraction and bacteria. Maximum zones were observed for ethyl acetate fraction (neutral) against Escherichia coli and Actenobactor baumannii with zone of inhibition 19.5 mm and 17.0 mm, respectively almost equal to the control. Their chemical composition was also analyzed by using FTIR spectroscopy and spot test. These fractions and crude were found containing amides, amino acids, amines, phenols, alcohols and aldehydes functional groups. Their antibacterial activity was then correlated with their chemical composition along with their determination for percent yield and percentage of extraction.

Page(s): 793-801

DOI: DOI not available

Published: Journal: Journal of Chemical Society of Pakistan, Volume: 39, Issue: 5, Year: 2017

Keywords:

antimicrobial activity , FTIR spectroscopy , waste fish scales , agar well diffusion method

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