Abstract:
Mobile genetic elements (MGEs) or transposable elements (TEs) are the most abundant mobile elements actively proliferating in various genomes contributing to their genome size duplication, evolution, diversification, and plasticity. The current work focused on the identification, molecular characterization and diversification of MGEs in selected plant genomes using bioinformatics, molecular and cytogenetic techniques. Various MGEs belonging to different superfamilies were identified by using different softwares like LTR_Finder, LTR_STRUC, JDotter, MITEHunter and many others softwares. LTR retrotransposons like Copia, Gypsy, Non-LTR retrotransposons SINES and LINES predominated, while DNA transposons were represented by CACTA, hAT, Harbinger, Mariner, Mutator and MITEs superfamilies. The Helitron, Politron and Maverik elements were rarely observed in these plants. PCR amplification of reverse transcriptase (RT) of Retrotransposons and transposase (TNP) of DNA transposons revealed their distribution among various plants (Brassica, Banana) genomes, with some elements were found to be specie or genera specific, while others were mobilized by horizontal transfer. The fluorescent in situ hybridization (FISH) methodologies confirmed the random and variable distribution of these elements on chromosomes of various species. The evolutionary relationship of these elements resolved them into superfamily and family specific lineages, while others clustered together showing homology in their sequences. The results enabled the characterization, annotation, evolutionary dynamics and understanding of structural features of full length MGEs and their derivatives in Brassica, Banana, and Date palm genomes.
Page(s):
0-0
DOI:
DOI not available
Published:
Journal: First International Conference on Revamped Scientific Outlook of 21st Century (Abstract Book), Volume: 0, Issue: 0, Year: 2022
Keywords:
evolution
,
genome
,
Gypsy
,
DNA transposons
,
Brassica
,
bioinformatics