Transcriptome Analysis of Apoptotic Markers in Peripheral Blood Mononuclear Cells of Hcv Genotype-3a Infected Patients. | [Journal of Postgraduate Medical Institute • 2018]

Author(s):

1. Afsheen Zakir: Department of Physiology and Cell Biology, University of Health Sciences, Lahore - Pakistan

2. Saba Khaliq: Department of Physiology and Cell Biology, University of Health Sciences, Lahore - Pakistan

3. Shah Jahan: Department of Physiology and Cell Biology, University of Health Sciences, Lahore - Pakistan

4. Khalid Pervaiz Lone: Department of Physiology and Cell Biology, University of Health Sciences, Lahore - Pakistan

Abstract:

Objective: To examine the HCV genotype 3a specific effects on caspases involved in apoptosis, expression levels of caspases-3, 8, 9, and 10 in peripheral blood mononuclear cells (PBMCs) of HCV genotype 3a infected patients. Methodology: This hospital based case controlled study was carried out between May 2014 to March 2015 on 72 subjects (36 HCV cases and 36 healthy controls). Blood samples of already diagnosed HCV patients of genotype 3a who were not on any antiviral therapy and HCV negative controls were collected. After extracting Ribonucleic acid (RNA) from samples it was reverse transcribed into complementary deoxyribonucleic acid (cDNA). Relative gene expression was performed for caspases-3, 8, 9 & 10. Results: Overall alanine transaminase and aspartate transaminase (ALT and AST) were raised, i.e. 85.20 ±40.64 and 97.43 ±2.77 in HCV infected subjects, as compared to controls 54 ±29.5 and 33 ±18.3 respectively (p <0.001). Gene expression analysis exhibited a statistically significant increase of 3.77, 3.74, 4.36 and 2.86 folds of caspases-3, 8, 9 & 10 respectively (p ?0.001) in HCV patients.

Page(s): 123-127

DOI: DOI not available

Published: Journal: Journal of Postgraduate Medical Institute, Volume: 32, Issue: 2, Year: 2018

Keywords:

Keywords are not available for this article.

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