Abstract:
Mycotoxins are toxic natural food contaminants, mainly produced by moulds of genera Aspergillus, Penicillum and Fusarium. Cereal grains are the most suspected food material, which can be contaminated by mycotoxins. Since cereals are being consumed worldwide in a bulk, so contaminated cereals poses a high risk to general public health.One of the important aspect which could significantly contribute to manage mycotoxins contamination, is the proper analysis and surveillance of food. There is a dire need of surveillance of mycotoxin contamination in food consumed by Pakistani population. Initial screening by High Performance Liquid Chromatography (HPLC), revealed a high-level of aflatoxins contamination in Pakistani cereals. Since HPLC method is non-confirmatory and may give a false positive results, the analytical method was upgraded to confirmatory one using liquid chromatography-tandem mass spectrometric technique. Mycotoxin contamination profile of Pakistani food (rice, maize, wheat and maize products) using a multi-mycotoxin LC-MS/MS methodology, has been investigated, with a subsequent risk assessment of mycotoxins exposure to Pakistani population. Myco-flora of contaminated cereal posing high risk (rice) was isolated and identified. Inhibition of prevalent fungus (Aspergillus flavus) and its toxigenic potential associated with rice, using natural inhibitor was investigated. Comparison of eight essential oils by their chemical composition, anti-fungal activity and anti-aflatoxigenic effect was done. The study also investigate an eco-friendly and inexpensive approach to detoxify Aflatoxin B1 in real food medium using UV and sunlight sources with optimization of different variables like sample thickness, time of exposure and wavelength of applied light source. Structural identification of degraded products with possible paths of generated fragments ions by MS/MS was proposed and biological toxicity assessment of degraded products compared to parent compound was evaluated
Page(s):
117-117
DOI:
DOI not available
Published:
Journal: Abstract Book on Global Science Technology and Management Conference, Volume: 0, Issue: 0, Year: 2023
Keywords:
Inhibition
,
Degradation
,
Foodstuff
,
Mycotoxins