Abstract:
In recent years, various types of metal and metal-oxides nanoparticles attained copious interest as an alternate method for the management of plant diseases. The current investigation was aimed at the green synthesis of nanoparticles to induce resistance in tomato plants against fusarium wilt. Green synthesized nanoparticles via MA-AgNPs, CF-CuONPs and BC-IONPs were characterized and used to investigate the in vitro and in vivo antifungal activity at various concentrations. In-vitro antifungal potential of all three types of nanoparticles expressively inhibited mycelial growth and spore germination of Fusarium oxysporum f.sp. lycopersici in a dose-dependent manner. The highest percentage inhibition in mycelial radial growth (96.8 ± 0.23%) and decline in spore germination rate (4.67%) was observed at 140 µg/mL of MA-AgNPs in contrast to the control and fungicide treatment. Monographs of the Scanning electron microscope revealed the ultrastructural changes in fungal hyphae in response to higher concentrations of nanoparticles signifying the detrimental effect of these NPs on the fungal mycelial surface. DCFH-DA fluorescence revealed ROS accumulation in fungal mycelium by showing strong green colour after treatment with studied NPs however, insignificant to very weak fluorescence was observed in control samples. Each type of nanoparticle was further characterized to get information about wavelength range, functional nature, crystallographic structure, size, shape and stability. The microwave-assisted MA-AgNPs showed a peak at 434 nm by using 5 mL of M. azedarach leaf extract and 2.5 mM of AgNO3 solution at pH 8, exposed to 30 s of microwave irradiations. MA-AgNPs indicated stability even after six months. Spherical shaped nanoparticles ranged from 12-46 nm were confirmed by XRD, SEM and TEM analysis. MA-AgNPs indicates the negative zeta potential of -22.3 mV. In addition, uptake of these nanoparticles did not show any visible sign of toxicity on plant yield.
Page(s):
47-47
DOI:
DOI not available
Published:
Journal: Abstract Book on Second International Conference on Recent Approaches in Plant Sciences (RAPS-23) 4-5 May 2023 , Volume: 0, Issue: 0, Year: 2023
Keywords:
Plant extracts
,
nanotechnology
,
wilt
,
Fungi