Author(s):
1. Muhammad Asif Nawaz:
Department of Biotechnology, SBB University, Sheringal, Dir Upper, KPK, Pakistan
2. Sidra Pervez:
The KIBGE, University of Karachi, Karachi, Pakistan
3. Shah Ali Ul Qader:
The KIBGE, University of Karachi, Karachi, Pakistan
Abstract:
The biodegradable agro-industrial wastes are mostly considered as potential sustainable source for the production of various value-added products from microbial species. Due to easy availability and economical profitability, agro-industrial wastes are preferred for large-scale production of enzymes and also to improve microbial cell growth. The hydrolytic enzymes can selectively hydrolyze the internal linkages of complex carbohydrates to release glucose moieties which can be further utilized in different industrial bioprocess. In the current study, sweet potato peel (Ipomoea batatas) was observed as the most favorable substrate for the maximum synthesis of a-1, 4-glucosidase among
various agro-industrial wastes. Bacillus licheniformis KIBGE-IB4 produced maximum quantity of a- 1, 4-glucosidase when growth medium was supplemented with 1% substrate. It was evident from the results that bacterial isolate secreted high titer of a-1,4-glucosidase in the presence of peptone, yeast extract and meat extract with optimum concentration of 0.4%, 0.1% and 0.4% respectively. Bacillus
licheniformis KIBGE-IB4 revealed maximum enzyme productivity at 40°C and pH-7.0 after 48 hours of fermentation period. An improved and cost effective growth medium design resulted 570.63±28.53 U mg-1 of a-1,4-glucosidase from B. licheniformis KIBGE-IB4. This enzyme can be used to fulfill the accelerating demand of food and pharmaceutical industries. Further purification and immobilization of this enzyme can also enhance its utility for various bioprocesses.
Page(s):
210-210
DOI:
DOI not available
Published:
Journal: Abstract Book on International Conference on Food and Applied Sciences (ICFAS-23) 3-5 August 23, Volume: 0, Issue: 0, Year: 2023
Keywords:
optimization
,
production
,
Saccharification
,
Sweet potato peel
,
a1
,
Agroindustrial waste
,
4glucosidase
References:
References are not available for this document.
Citations
Citations are not available for this document.