Abstract:
Retinoblastoma related gene (rbr-4) which is a homologue of pRb and, a negative regulator of the cell cycle is one of such genesthat has been reported to have a role in the cell cycle, cell proliferation and genome integrity. Our data suggested rbr-4mutant leads to genome integrity and causes 25-30% triploidy in plants. The pollen count was reduced to 50-60 inrbr-4 mutant as compared to control col0,as opposed to~400 pollens are in freshly opened flowers. DAPI staining of pollen revealed that both mitotic and meiotic processes are disturbed, which leads to abnormal development of pollen. Similarly, seed abortions were observed in 80-84% of embryos and seeds failed to reach the final stages of viability. Similarly, a 42% reduction in silique length were observed. Pollen tube germination assay revealed 50% abnormal growth. Transmission electron microscopy revealed abnormal pollen walls, accumulation of oil bodies and starch in pollen grains. Transcriptomic data revealed that some salt-stressed responsive genes Kin12A, Kin12B, BT2,and BT3 genes were downregulated in seedlings of the rbr-4 mutant. Our study has concluded that the retinoblastoma related (rbr-4) gene is an important transcriptional factor responsible both for development of male and female germline, seeds, silique, and pollen tube, as well as normal fertilization of the plants.
Page(s):
105-105
DOI:
DOI not available
Published:
Journal: Abstract Book on International Conference on Food and Applied Sciences (ICFAS-23) 3-5 August 23, Volume: 0, Issue: 0, Year: 2023
Keywords:
retinoblastoma
,
Ploidy
,
Pollen tube
,
Genome integrity
,
DAPI