A new RP-HPLC method for monitoring of atenolol: Application to atenolol metal interactions studies. | [Journal of Chemical Society of Pakistan • 2008]

Author(s):

1. Najma Sultana: Research Institute of Pharmaceutical Sciences, Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Karachi, Pakistan

2. Benish Iftikhar: Research Institute of Pharmaceutical Sciences, Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Karachi, Pakistan

3. Muhammad Saeed: Department of Chemistry, University of Karachi, Karachi, Pakistan

4. Muhammad Nawaz: Department of Chemistry, University of Karachi, Karachi, Pakistan

Abstract:

A sensitive high-performance liquid chromatography method was developed for the determination of atenolol in bulk drug and pharmaceutical formulation in the presence offlurbiprofen as internal standard. Separation was carried out on a Purospher Start, C18 (5µm, 250 x 4.6 mm) column with MeOH: H2O (80: 20, % v/ v) solvent systems and pH was adjusted to 2.85 with ortho-phosphoric acid. Mobile phase was pumped with a flow rate ofI ml/ minute with UV detection at 224 nm. Standard curves were linear over the concentration range of 0.25-30 µg / ml. The inter-day and intra-day coefficients of variation were less than 1 % at different concentrations. The method was then applied to study interaction of atenolol with essential and trace elements. The combination of hydroxy and aminogroups makes atenolol a good chelating ligand. Drug metal interaction studies were carried out at 37°C to monitor the complexation ofdrug with metal ions. The order of complexation of atenolol with metals was found to be copper> ferric> chromium> zinc > ferrous> cobalt> nickle> magnesium> manganese> cadmium > calcium.

Page(s): 113-118

DOI: DOI not available

Published: Journal: Journal of Chemical Society of Pakistan, Volume: 30, Issue: 1, Year: 2008

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