Abstract:
Infectious bursal disease virus (IBDV) from field outbreaks was purified by cesium chloride gradient centrifugation and collected at density of 1.33. The partially purified IBDV was serially propagated in specific pathogen free embryonated chicken eggs (ECEs). Intensity of lesions of IBDV was high from passage number 11 to 15 and nil after 23rd passage. Embryo mortality recorded was highest (100%) at passage number 6 and 7, started decreasing after serial passage and it was zero till passage number 15. All the field isolates were similar in growth pattern and minor differences were observed in geometric mean titers (GMT) of virus recovered from chorioallantoic membrane (CAM), allantoic fluid (AF) and embryo by reverse passive haemagglutination (RPHA) test. GMT values of IBDV in CAM, AF and embryo at passage number 6 were 194, 168.9 and 222.9 whereas after passage number 13 were 1552.1, 1351.2 and 1782.9 respectively. Twenty fourth passage of IBDV in ECEs (103.5 EID50/ml) was used as live attenuated vaccine in comparison with live commercial vaccine (Bursine-2 at recommended dose) in broiler chicks for immunization. Immune response measured by indirect haemagglutination (IHA) test at day 35 was higher (GMT 2896.3) than Bursine-2 (GMT 891.4).
Page(s):
30-34
DOI:
DOI not available
Published:
Journal: Pakistan Journal of Life and Social Sciences, Volume: 8, Issue: 1, Year: 2010