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Transcriptome Sequencing Reveals Genes Involved in Formation of Asiatic Hybrid Lily Cultivar 'easy Dance' Petal Spot.
Author(s):
1. Xiangfeng He: Beijing Collaborative Innovation Center for Eco-Environmental Improvement with Forestry and Fruit Trees, Beijing 102206, China: College of Landscape Architecture, Beijing University of Agriculture, Beijing 102206, China
2. Shufa Xu: Key Laboratory of Pollinating Insect Biology, Ministry of Agriculture, Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, China
3. Pingsheng Leng: College of Landscape Architecture, Beijing University of Agriculture, Beijing 102206, China
4. Wenhe Wang: College of Landscape Architecture, Beijing University of Agriculture, Beijing 102206, China
Abstract:
Petal spot is an important characteristic for ornamental flowers. However, fewer studies have been done to uncover the molecular formation mechanism of petal spot in Lilium sp. In our research transcriptome sequencing of Asiatic hybrid lily 'Easy Dance' flower with large brown spot was performed. Transcriptome of brown-pigmented interior tissue of spot region (SP) was compared with un-pigmented exterior tissue of spot region (SNP) and no spot region (NS) of flower to identify structural genes and regulatory genes involved in brown spot formation. The results revealed that 11,544 unigenes exhibited significantly differential expression between NS and SP, and 12,636 unigenes between SNP and SP. Functional enrichment analysis of differential expressed genes revealed several pathways possibly involved in spot formation in Lilium. Some of unigenes annotated as CHS, PAL, C4H, F3'H, F3'5'H, F3H, FLS, DFR and 3GT indicated higher expression in SP compared with NS and SNP. All expressions of carotenoid biosynthesis genes were down-regulated in spot. In addition, six transcription factors annotated as bHLH, R2R3-MYB or WD40, which regulated anthocyanin biosynthesis in spot, were identified by transcriptome sequencing and phylogenetic analysis. Our research will help to deepen the understanding of formation mechanism of petal spot in Lilium.
Page(s): 939-944
Published: Journal: International Journal of Agriculture and Biology, Volume: 20, Issue: 4, Year: 2018
Keywords:
Keywords are not available for this article.
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