The Regulatory Networks Conferred by IFN-? in the Kidney of Takifugu rubripes. | [International Journal of Agriculture and Biology • 2018]

Author(s):

1. Derong Kong: College of Fisheries and Life Science, Dalian Ocean University, Dalian, 116023, China

2. Jun Cui: College of Fisheries and Life Science, Dalian Ocean University, Dalian, 116023, China

3. Zhicheng Wang: College of Fisheries and Life Science, Dalian Ocean University, Dalian, 116023, China

4. Lin Zang: College of Fisheries and Life Science, Dalian Ocean University, Dalian, 116023, China

5. He Sun: College of Fisheries and Life Science, Dalian Ocean University, Dalian, 116023, China

6. Ziwen Hu: College of Fisheries and Life Science, Dalian Ocean University, Dalian, 116023, China

7. Xin Li: College of Fisheries and Life Science, Dalian Ocean University, Dalian, 116023, China

8. Xuemei Qiu: College of Fisheries and Life Science, Dalian Ocean University, Dalian, 116023, China

9. Chen Jiang: College of Fisheries and Life Science, Dalian Ocean University, Dalian, 116023, China

10. Haiying Liu: College of Marine Science and Environment, Dalian Ocean University, Dalian, 116023, China

11. Tao Zhang: Dalian Tianzheng Industrial Co. Ltd, Dalian, 116011, China

12. Shengcong Liu: Dalian Tianzheng Industrial Co. Ltd, Dalian, 116011, China

13. Zhiqiang Jiang: College of Fisheries and Life Science, Dalian Ocean University, Dalian, 116023, China

14. Xuesong Meng: Dalian Tianzheng Industrial Co. Ltd, Dalian, 116011, China

15. Xiuli Wang: College of Fisheries and Life Science, Dalian Ocean University, Dalian, 116023, China

Abstract:

Takifugu rubripies is one of the most important economic fishes. Recently, because of various infectious diseases, production of T. rubripies has been seriously hindered. Interferon gamma (IFN-?) is critical for innate and adaptive immunity in fish. Here, a comparative RNA-Seq profiling study of the kidney of T. rubripes was performed to investigate the expression differences between the kidney samples with (Ts) and without (Cs) IFN-? infection. A total of 109,140,188 raw reads were generated from the Ts and Cs samples, including 106,717,920 clean reads. Additionally, a total of 19,124 expressed genes were identified from the two samples. Among these expressed genes, 292 genes showed significantly differential expression including 171 up-regulated and 121 down-regulated genes in the Ts sample. The GO, KEGG pathway enrichment and hierarchical clustering analyses indicated that the G-protein coupled receptor, Cytokine-cytokine receptor interaction, JakSTAT signaling pathways were likely to be affected by IFN-?. In addition, the protein-protein interaction (PPI) result showed that the MAPK6 was located at the center of this regulatory network involving IFN-?, suggesting that IFN-? may activate the MAPK signaling pathway. These results demonstrate the IFN-?-related molecular mechanisms in the kidney of T. rubripes, provide a basis for application of the recombinant IFN-? protein as a vaccine candidate against pathogen infection in aquaculture, and provide candidates to enhance pathogen-resistance in T. rubripes.

Page(s): 2189-2195

DOI: 10.17957/IJAB/15.0758

Published: Journal: International Journal of Agriculture and Biology, Volume: 20, Issue: 10, Year: 2018

Keywords:

Keywords are not available for this article.

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