Treatment of bovine serum albumin with formaldehyde would result in cytotoxicity to SH-SY5Y cells. | [Pakistan Journal of Biochemistry and Molecular Biology • 2014]

Author(s):

1. Sajjad Haider Naqvi: Department of Biochemistry, University of Karachi, Karachi, Pakistan

2. Syed Faizan-Ul-Hassan Naqvil: Department of Biochemistry, University of Karachi, Karachi, Pakistan

3. Lu Yang: lnstitute of Biophysics, State and Key Laboratory of Brain and Cognitive Sciences, Chinese Academy of Sciences (CAS) Beijing, P. R. China

4. Iftikhar Haider Naqvi: Dow University of Health Sciences (DUHS), Karachi, Pakistan

5. Muhammad Farhan: Department of Biochemistry, University of Karachi, Karachi, Pakistan

6. Rongqiao Het: lnstitute of Biophysics, State and Key Laboratory of Brain and Cognitive Sciences, Chinese Academy of Sciences (CAS) Beijing, P. R. China

Abstract:

Bovine Serum Albumin (BSA) is a well know globular protein having the tendency to form aggregates in the macromolecular assemblies. Its three dimensional structure is composed of three domains, each one is formed by six helices, and its secondary structure is essentially alpha helix. The structure of BSA shows stability at room temperature but as temperature increases results in formation of soluble aggregates through disulphide and non-covalent bonds. The simplest of the aldehydes is formaldehyde (FA). It is a bioactive and fatal compound, naturally present in human habitation. Formaldehyde, existing in all kinds of cells, may have some different and unknown physiological functions and may be related with some substantial human diseases and their pathology. The possible role of formaldehyde i n the formation of beta-amyloid aggregation related to the pathology of Alzheimer`s disease (AD) has been reported. The research further showed the high expression of SSAO co-localized with A beta deposits on the blood vessels in AD brains. Important findings of the study include the significant decrease in intrinsic fluorescence of protein with reference to control BSA both in a concentration and time dependent manner. SDS PAGE results showed some aggregation but there is no significant difference between the control and FA treated group. The important finding of the study is that the BSA monomer is highly cytotoxic to SH-SY5Y Cells. There is significant and higher level of cell cytotoxicity observed in SH-SY5Y cells for both 0 day and 7 days incubation with samples of BSA treated with formaldehyde as compared to BSA and formaldehyde alone. The results of the present study reveals that the BSA treated with formaldehyde would be transformed into cytotoxic protein. However further studies are requi red to investigate the possible role of lead in affecting the structure and function of proteins.

Page(s): 111-117

DOI: DOI not available

Published: Journal: Pakistan Journal of Biochemistry and Molecular Biology , Volume: 47, Issue: 1--2, Year: 2014

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