Author(s):
1. Rifat Jahan:
Department of Biochemistry, SBBWU, Peshawar, Pakistan; Department of Chemistry, Islamia College University, Peshawar, Pakistan
2. Mohammad Yousaf:
Department of Biochemistry, SBBWU, Peshawar, Pakistan.
3. Hamayun Khan:
Department of Biochemistry, SBBWU, Peshawar, Pakistan.
Abstract:
Background: Alzhimer's disease (AD) is globally affecting people and is one of the most prevalent cause of dementia. Still efficient treatment is not available for the patients so new compounds that are biologically active are regularly tested. It was, therefore, hypothesized to study the neuroprotective potential of Zinc Ortho Methyl Carbonodithioate (thereafter called ZOMEC) in induced AD model using adult albino mice. Methodology: Scopolamine was used to induce Alzhimer's disease in the mice. The mice were injected for five weeks. Mice were divided into four groups. ZOMEC (30 mg/Kg) was administered into two groups of mice for last three weeks on daily basis. One of the group received 0.9% saline and the other group was injected with SCOP (1 mg/Kg) for initial two weeks. The other two groups of mice received 0.9% saline (Control group) and SCOP (1 mg/Kg) respectively for five weeks. After memory related behavioral analysis the brain homogenates were evaluated for the antioxidant potential of ZOMEC and multiple protein markers were examined through western blotting. Results:The resultsshowed that ZOMEC decrease oxidative stress by increasing catalase (CAT) and glutathione S transferase (GST) and decreasing the lipid peroxidation (LPO). The SIRT1 and pre and post synaptic marker proteins, synaptophysin (SYP) as well as post synaptic density protein (PSD-95) expression were also enhanced upon ZOMEC treatment. Furthermore, memory impairment was rescued and ZOMEC appreciably abrogated the Aß accumulation, BACE1 expression and the p-JNK pathway. The inflammatory protein markers, NFkß and IL-1ß in ZOMEC treated mice were also comparable with control group. The predicted interaction of ZOMEC with SIRT1 was further confirmed by molecular docking. Conclusion: These findings thus provide initial reports on efficacy of ZOMEC in SCOP-induced AD model.
Page(s):
70-70
DOI:
DOI not available
Published:
Journal: Abstract Book on International Conference on Food and Applied Sciences (ICFAS-23) 3-5 August 23, Volume: 0, Issue: 0, Year: 2023
Keywords:
Alzheimers Disease
,
Sirt1
,
SYP
,
pJNK
,
PSD95
References:
References are not available for this document.
Citations
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