Transmission of hepatitis-b virus through salivary blood group antigens in saliva. | [Journal of College of Physicians and Surgeons--Pakistan : JCPSP • 2010]

Author(s):

1. Sultan Ayoub Meo: Department of Physiology/Medicine, College of Medicine, King Khalid University Hopital, King Saud University, Riyadh, KSA

2. Ayman Assad Assad: Department of Hepatology Division, College of Medicine, King Khalid University Hopital, King Saud University, Riyadh, KSA

3. Faisal M Sanie: Department of Medicine, Hepatology Division, Military Hospital, Riyadh, KSA

4. Nawab Din Baksh: Department of Biochemistry, College of Medicine, King Khalid University Hopital, King Saud University, Riyadh, KSA

5. Ahmed Al-Qahtani: Biological and Medical Research Center, King Faisal Specialist Hospital and Research Center, Riyadh, KSA

6. Zahoor Ali Shaikh: Department of Physiology/Medicine, College of Medicine, King Khalid University Hopital, King Saud University, Riyadh, KSA

Abstract:

Objective: To determine an association between transmission of hepatitis B virus and secretor and non-secretor status of salivary blood group antigens. Study Design: Cross-sectional, analytical study. Place and Duration of Study: The Department of Physiology and Division of Hepatology, College of Medicine, King Khalid University Hospital, King Saud University, Riyadh, Kingdom of Saudi Arabia, from 2007 to 2009. Methodology: Eighty eight known patients, who were positive for Hepatitis B Surface Antigen [HBsAg] were recruited. Saliva was collected for investigating the secretor and non-secretor status by using blood typing kit number Kemtec Educational Science USA. Hepatitis B Surface antigen test was performed on Enzyme Linked Immunosorbent Assay technique. Polymerase chain reaction [PCR] on saliva was also carried out in High Performance Thermal Cycler-Palm- Cycler- [Corbett Life Science, Sydney, Australia] and enzymatic amplification of extracted viral DNA was performed using primers covering the promoter of the core region of HBV. Results: Out of the 88 subjects, 61 belong to blood group O, 20 to A and 7 subjects to blood group B. Fifty subjects were secretors [salivary blood group antigens positive] and 38 subjects were non-secretors [salivary blood group antigens negative]. Among core gene positive 25 (69.4%) were secretors and 11 (30.6%) were non-secretors. However, in core gene negative 25 (48.1%) were secretors and 27 (51.9%) were non-secretors. Conclusion: The result shows an association [p=0.047] between secretor and non-secretors status of the salivary blood group antigens with core gene positive and core gene negative.

Page(s): 444-448

DOI: DOI not available

Published: Journal: Journal of College of Physicians and Surgeons--Pakistan : JCPSP, Volume: 20, Issue: 7, Year: 2010

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