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Trends in Frequency, Potential Risks and Antibiogram of E. coli Isolated from Semi-Intensive Dairy Systems
Author(s):
1. Muhammad Ahsan Anwar: Department of Clinical Medicine and Surgery, University of Agriculture,Faisalabad,Pakistan
2. Sidra Aziz: Department of Clinical Medicine and Surgery, University of Agriculture,Faisalabad, Pakistan
3. Khurram Ashfaq: Department of Clinical Medicine and Surgery, University of Agriculture,Faisalabad, Pakistan
4. Amjad Islam Aqib: Department of Medicine, Cholistan University of Veterinary and Animal Sciences,Bahawalpur,Pakistan
5. Muhammad Shoaib: Key Laboratory of New Animal Drug Project, Gansu Province/Key Laboratory of Veterinary Pharmaceutical Development, Ministry of Agriculture and Rural Affairs/ Lanzhou ; Institute of Husbandry and Pharmaceutical Sciences of CAAS, Lanzhou, PR China
6. Muhammad Aamir Naseer: Department of Clinical Medicine and Surgery, University of Agriculture,Faisalabad,Pakistan
7. Mughees Aizaz Alvi: Department of Clinical Medicine and Surgery, University of Agriculture,Faisalabad,Pakistan
8. Iqra Muzammil: Department of Veterinary Medicine, University of Veterinary and Animal Sciences,Lahore,Pakistan
9. Zeeshan Ahmad Bhutta: Department of Clinical Medicine and Surgery, University of Agriculture,Faisalabad, Pakistan; Laboratory of Biochemistry and Immunology, College of Veterinary Medicine, Chungbuk National University, Cheongju, Chungbuk, 28644, Republic of Korea;
10. Huma Sattar: Institute of Molecular Biology and Biotechnology, The University of Lahore,Pakistan
11. Arslan Saleem: School of Engineering and Design Technische Universität München, Arcisstraße München, Germany
12. Tean Zaheer: Department of Parasitology, University of Agriculture,Faisalabad,Pakistan
13. Fakhara Khanum: National Institute of Food Science and Technology, University of Agriculture Faisalabad, Pakistan
14. Asif Mahmood: Veterinary Research Institute Lahore, Punjab, Pakistan
Abstract:
Semi-intensive dairy systems are prevailing in tropical and subtropical countries that need to be probed for public health concern pathogens. The current study was designed to map trends in prevalence of dairy-originated E. coli, associated risk factors, and altering in-vitro drug susceptibility patterns. A total of n=200 subclinical mastitis milk samples were intended to be collected using purposive sampling method from semi-intensive dairy systems for which n=764 dairy animals (n=440 buffaloes, n=324 cattle) were screened. Standard biochemical and microbiological methods coupled with polymerase chain reaction (23sRNA) were applied to identify E. coli from subclinical milk samples. The study, overall, found a 26.18% (200/764) prevalence of subclinical mastitis and 13.50% (27/200) E. coli from subclinical samples. Among assumed risk factors, lack of use of teat dip (OR=8.26, C.I. = 2.73-24.91), higher age groups (OR=17.87, C.I. = 4.42-72.16), parity number >3 (OR=3.68, C.I. = 1.59-8.49), underweight animals (OR=2.89, C.I. = 1.11-7.53), and mid-lactation (OR=14.94, C.I. = 3.04-73.24) were dominant potential risk factors for E. coli infection. Antibiogram showed 42.86 and 21.43% of E. coli isolates resistant to amoxicillin-clavulanate and oxytetracycline, respectively. It was noted that more than 40% (42.86, 60.87, 57.89, 66.86, and 67.86%) of E. coli fall in intermediate susceptible cadre against 62.5% of tested antibiotics. In conclusion, increasing percentages of E. coli, higher number of potential risk factors, and antibiotic susceptibility inclining towards resistance demands stern compliance in anticipated time to avoid any grave situation.
Page(s): 167-172
Published: Journal: Pakistan Veterinary Journal, Volume: 42, Issue: 2, Year: 2022
Keywords:
Antibiogram Dairy milk E coli Risk factors Semiintensive dairy systems
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