Abstract:
Obesity is one of the most prevalent global health issues and a major risk factor for non-communicable diseases such as diabetes, dyslipidemia, hypertension, and cardiovascular disorders, thereby increasing morbidity and mortality. According to the World Health Organization, obesity incidence has tripled since1975. Obese individuals show elevated levels of acute phase proteins, pro inflammatory mediators, and circulating cytokines, predisposing them to autoimmune and inflammatory diseases. This study investigated the correlation between the IL-17F rs23977084 variant and obesity using a case-control design, while also identifying the most damaging ns SNPs with bioinformatics tools. Blood samples from 200 participants (100 obese, 100 controls) were collected, and DNA was extracted using the phenol-chloroform method. Genotypic and allelic distributions of IL-17F SNP (rs2397084) were analyzed via ARMS-PCR. Results indicated no significant difference in genotype distribution (P=0.5698,?2 =1.125) or allele frequency between cases and controls, suggesting that this SNP is not strongly associated with obesity risk. Advanced bioinformatics platforms, including SNPs & GO, SIFT, PolyPhen2, PHD SNP, and Panther, were used to identify deleterious ns SNPs in IL-17F. Additional validation of protein stability, structure, and functional impact was performed using I-Mutant, Mut Pred, and Con Surf. Three-dimensional protein models were generated with I-TASSER and visualized using Chimera v1.11, while RMSD and TM-scores were predicted using TM-align. Gene-gene interaction networks were also constructed. Several damaging ns SNPs were identified, including R77S, R77C,S78Y, P81S, D89H, R92G, R92W, R92Q, A100T, A100V, C107R, G112R,I123T, V129A, R131W, R132S, C152R, C152Y, C152F, T153A, T153I,V155G, and P157L. These variants may cause significant structural and functional defects in IL-17F, potentially contributing to obesity and autoimmune disorders.
Page(s):
122-122
DOI:
DOI not available
Published:
Journal: 1st International Conference on "Recent Advances in Green Biotechnology and Climate Resilience", September 15-16, 2025, Volume: 1, Issue: 1, Year: 2025