Abstract:
Photodynamic Antimicrobial Chemotherapy (PACT) is a relatively new method that utilizes nontoxic substancesphotosensitizers, visible or ultraviolet light and cytotoxic reactive oxygen species (ROS), in order to give a phototoxic response, normally via oxidative damage. A wide range of photosensitizers, both natural and synthetic, is available with differing physicochemical make-up and light-absorption properties. PACT uses various photosensitizers, and, out of these, zinc phthalocyanine (ZnPc)-based photosensitizer are of particular interest for their unique physicochemical properties. PACT has considerably advanced with new light sources, biocompatible photosensitizers, bioconjugate methods, and efficient ROS production. The PACT technology has evolved to compete with or replace antibiotics, reducing the burden of antibiotic resistance. This technique has been found to be effective against a wide spectrum of microorganisms, including bacteria, viruses, and fungi. Of greater interest is their ability to destroy resistant strains of microorganisms and in effect help in combating the emergence of antimicrobial resistance. In the current study, we synthesized zinc phthalocyanine (ZnPC) conjugated with antimicrobial peptide (AMP) protected via Wang resinfor photoinactivation assay to enhance the bacterial killing efficacy of the peptide upon illumination under red light. Various strain of bacteria such as Gram-positive Staphylococcus and Gram-negative E. Coli isolated were treated in vitro with already synthesized compound under different conditions. The (AMP) showed selectivity toward the Gram-positive Staphylococcus strain of bacteria. We observed that the conjugate also displayed a photoinactivation effect against the Gram-positive strains of S. aureus. On other hand AMP is not selective toward Gram-negative E. Coli before and after conjugation. Then we used potassium iodide (KI) to enhance the photodynamic effect of conjugate, so that the KI react with free radical to generate some cytotoxic iodide radical which can kill Gram-negative E. Coli more efficiently. In light of this study, further research into the validity of PACT, coupled with the photosensitizers should be conducted in order to potentially develop alternative antimicrobial treatment.
Page(s):
182-182
DOI:
DOI not available
Published:
Journal: Abstract Book on International Conference on Food and Applied Sciences (ICFAS-23) 3-5 August 23, Volume: 0, Issue: 0, Year: 2023