The metagenomic analysis of potential pathogenic emerging bacteria in fleas | [Pakistan Journal of Biological Sciences • 2021]

Author(s):

1. Dina Victoria Rombot: Faculty of Medicine, Sam Ratulangi University, Manado, North Sulawesi, Indonesia

2. Mokosuli Yermia Semuel: Department of Biology, Faculty of Mathematics and Natural Sciences, Manado State University, Tondano, North Sulawesi, Indonesia;Laboratory of Biomolecular and Bioactivity, Faculty of Mathematics and Natural Sciences, Manado State University, Tondano, North Sulawesi, Indonesia

Abstract:

Background and Objective: At present many pathogenic microbes that cause disease in humans are transmitted through animals. Ctenocephalides felis is specific ectoparasites in cats. Metagenomic research on the digestive tract and body surface of C. felis has been conducted. DNA genomics was extracted from the body surface and digestive tract of C. felis. Materials and Methods: Metagenomic analysis has used the 16S rRNA gene (V3-V4 region). Sequencing was carried out using New Generation Sequencing at the First BASE Laboratory, Singapore. Results: Wolbachia has the most significant bacterial composition in C. felis (94.4%), we were found bacteria with a composition >1% that have never been reported to be associated with C. felis. Also, there were 0.2% of bacteria whose taxonomic status cannot be determined. Conclusion: The results of this study become a vital reference pathogenic bacteria that can be transmitted to humans and animals through C. felis. It is necessary to study the resistance of bacteria isolated from C. felis to antibiotics in the future

Page(s): 1084-1090

DOI: 10.3923/pjbs.2021.1084.1090

Published: Journal: Pakistan Journal of Biological Sciences, Volume: 24, Issue: 10, Year: 2021

Keywords:

staphylococcaceae , bacteria composition , 16S rRNA , caulobacteriaceae , Metagenomics , symbiotic , Ctenocephalides felis

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