Author(s):
1. Masooma Batool Shahzadi:
Department of Bioinformatics and Biotechnology, Government College University Faisalabad
2. Faiz-ul-Hassan:
Institute of Animal and Dairy Sciences, University of Agriculture Faisalabad
3. Muhammad Saif ur Rehman:
Institute of Animal and Dairy Sciences, University of Agriculture Faisalabad
4. Muhammad Shah Nawaz ul Rehman:
Center for Agricultural Biochemistry and Biotechnology, University of Agriculture Faisalabad, Pakistan
5. Nazia Nahid:
Department of Bioinformatics and Biotechnology, Government College University Faisalabad
Abstract:
Anaerobic microbes present in the rumen fluid play a critical role in the digestion of feed. Ruminants get energy from metabolic conversion of their feed by the action of these microbes. Among themthe lactic acid bacteria (LAB) are involved in the digestion with a potential probiotic impact. Majority of the probiotics used in Pakistan are being imported from other countries. The isolation of native microbiota with potential probiotic impact is important for a durable and adaptive response. In this study 1 mL rumen fluid was collected from the 3 ruminally cannulated Nili Ravi bulls weighing about 350 kg body weight at an age of 5-6 years being fed on the green millet fodder. 10-fold serial dilutions were made in phosphate-buffered saline (PBS) for each samples and 0.2mL dilution was plated onto de Man, Rogosa and Sharpe (MRS) media. Plates were incubated at 37oC for 48 hours for isolation of LAB. 50 creamy white colonies were selected randomly and grown separately in MRS broth. All selected isolates were Gram positive and catalase negative, a characteristic of LAB. Total genomic DNA was extracted by a modified CTAB method for the molecular identification of LAB. A PCR fragment of about 1700bp was amplified using the 16S rRNA primers 8F (5'-AGAGTTTGATCCTGGCTCAG-3') and 1541R (5'-AAGGAGGTGATCCAGCCGCA-3'). Sequence analysis of 16S rRNA fragment revealed that isolated bacteria were 96-98%identical to the Weissella, Paenibacillus, and Pediococcus sp. The phylogenetic analysis showed that the bacterial isolates from rumen predominantly showed identity to the probiotic bacteria Weissella confusa, Paenibacillus barcinonensis, and Pediococcus acidilactic. This research aided in the isolation of LAB from rumen fluid that can be a promising contender for fodder fermentation in the silage sector and native probiotics to treat enteric pathogenic bacteria.
Page(s):
171-171
DOI:
DOI not available
Published:
Journal: Abstract Book on Global Science Technology and Management Conference, Volume: 0, Issue: 0, Year: 2023
Keywords:
lactic acid bacteria
,
Rumen
,
NiliRavi Buffalo
,
16S rRNA
References:
References are not available for this document.
Citations
Citations are not available for this document.