Uptake and transport characteristics of organic and inorganic nitrogen in rice seedlings | [Pakistan Journal of Botany • 2023]

Author(s):

1. ZHAOHUI ZOU: Hunan Academy of Agricultural Science,Changsha 410125,PR China

2. XIAN LI: Hunan Academy of Agricultural Science,Changsha 410125,PR China

3. GANGQIAO DENG: Hunan Academy of Agricultural Science,Changsha 410125,PR China

4. HONGKE XIE: Hunan Academy of Agricultural Science,Changsha 410125,PR China

5. YONG ZHANG: Hunan Academy of Agricultural Science,Changsha 410125,PR China

6. JUN LIU: Medical College, University of South China,Hengyang 421001,PR China; The Key Laboratory of Hengyang City on Ecological Impedance Technology of Heavy Metal Pollution in Cultivated Soil of Nonferrous Metal Mining Area,Hengyang 421001,PR China

7. YIJIE ZHOU: Hunan Academy of Agricultural Science,Changsha 410125,PR China

8. XIAOYI TU: Hunan Academy of Agricultural Science,Changsha 410125,PR China

9. AIGUO HE: Hunan Academy of Agricultural Science,Changsha 410125,PR China

Abstract:

The study was conducted in sterile hydroponics using isotopes tracer technique to demonstrate the posibility for rice seedlings (variety: C Liangyou 266) to directly absorb and utilize molecular organic nitrogen, transport and assimilation of organic/inorganic nitrogen. Further the activity of assimilation-related enzymes i.e Glutamic-oxaloacetic transaminase (GOT), Glutamic-pyruvic transaminase (GPT) and Glutamate dehydrogenase (GDH), in rice seedlings were also studied using isotopic tracing technique. In this regard 15N-ammonium sulfate, 15N-potassium nitrate and 2-13C-15N-glycine salts were used to analyzes the abundance of 13C/15N. The results suggest that the 15N excess and the ratio of 15N excess in shoot to root under glycine nitrogen treatment (Gly-N) were significantly higher than those under ammonium nitrogen or nitrate nitrogen treatment (p<0.05); the 13C excess/15N excess ratios of rice organs and the whole seedling measured at 24h after Gly-N approximated the theoretical value of 1:1, which showed gradual declines 48h and 72h later. The activities of glutamic-oxaloacetic transaminase (GOT), glutamic-pyruvic transaminase (GPT) and glutamate dehydrogenase (GDH), in rice seedlings cultured by organic nitrogen were significantly higher than those by inorganic nitrogen (p<0.05). The findings also showed that rice seedlings are capable for direct absorbing and utilizing molecular glycine nitrogen. The absorption rate of glycine nitrogen was much higher than that of ammonium nitrogen or nitrate nitrogen. The transport capabilities of organic and inorganic nitrogen in rice plants, ranked in descending order are as follows: glycine nitrogen > ammonium nitrogen > nitrate nitrogen.

Page(s): 337-343

DOI: 10.30848/PJB2023-1(36)

Published: Journal: Pakistan Journal of Botany, Volume: 55, Issue: 1, Year: 2023

Keywords:

Rice , mechanism , Absorption and transport , 13C15Nglycine

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