Alginate production by a mutant strain of Aotobacter vinelandii using shake flask fermentation | [Pakistan Journal of Botany • 2011]

Author(s):

1. Zahid Ali Butt: Institute of Industrial Biotechnology, Government College University, Lahore, PAKISTAN

2. Ikram-Ul-Haq: Institute of Industrial Biotechnology, Government College University, Lahore, PAKISTAN

3. Muhammad Abdul-Qadeer: Institute of Industrial Biotechnology, Government College University, Lahore, PAKISTAN

Abstract:

The present study describes the improvement of parent strain of Azotobacter vinelandii NRRL-1 4641 for enhanced alginate production using shake flask fermentation. Results indicated that a mutant A. vinelandii EMS-45 obtained after EMS treatment gave 1.55 fold better alginate productions than that of the parent strain. Different cultural conditions were employed to optimize the polymer production in the fermentation broth. It was noted that maximum alginate production (6.17 g/l) was obtained with nitrogen rich phosphate limited medium (NRP) after 110 h of incubation period. The incubation temperature 30°C, pH 7.0, agitation intensity 200 rpm, carbon and nitrogen source were also optimized and sucrose and peptone were found as the best to support 1.55 fold higher alginate than that in un-optimized medium.

Page(s): 1053-1067

DOI: DOI not available

Published: Journal: Pakistan Journal of Botany, Volume: 43, Issue: 2, Year: 2011

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