Altered proliferative response of normal and malignant cells exposed to a polysaccharide extract from Tamarindus indica Pulp. | [Journal of Medical Science • 2005]

Author(s):

1. Fernando Garcia-Tamayo: Departamento de Biologia, Facultad de Quimica, Universidad Nacional Autonoma de Mexico, Mexico D.F, 04510 Mexico

2. Guadalupe Reyes: Departamento de Biologia, Facultad de Quimica, Universidad Nacional Autonoma de Mexico, Mexico D.F, 04510 Mexico

3. Teresa Izquierdo: Departamento de Sistemas Biolbgicos, Universidad Autonoma Metropolitana-Xochimilco, Mexico D.F, Mexico

4. Patricia Elizalde: Departamento de Quimica Organica, Facultad de Quimica, UNAM, Mexico, D.F, 04510 Mexico

5. Elba Rojas: Departamento de Quimica Organica, Facultad de Quimica, UNAM, Mexico, D.F, 04510 Mexico

6. Emilio Rojas Del Castillo: Departamento de Genetica y Toxicologia Ambiental, Instituto de Investigaciones Biomedicas, UNAM, Mexico, D.F, 04510 Mexico

7. Luis I. Terrazas: Unidad de Biomedicina, Facultad de Estudios Superiores-Iztacala, UNAM, Tlalnepantla, Edo. de Mexico, 54090 Mexico

Abstract:

A water-soluble Polysaccharide Extract (PE) isolated from the pulp of the fruit bodies of a Mexican species of Tamarindus indica L. was characterized by thin-layer chromatography and HPLC. Fructose, glucose, arabinose and xylose were the major components of the hydrolyzed PE samples. Normal lymphocytes and several lineages of malignant cells were cultured in presence of different amounts of PE solution and their effects on cellular proliferation were assessed by tritiated thymidine uptake. After incubation for 48 h, a significant reduction on the thymidine uptake was observed when Con-A-stimulated mice’s splenocytes were incubated with tamarind PE. Furthermore, a similar reduction in proliferative activity was detected also in CTLL-2 and HeLa cell lines. A comparative control assay showed a more intense inhibition when similar amounts (5-100 µg mL-1) of cis-platinum was added to HeLa cells. The morphological examination of PE treated HeLa cells revealed their transformation into multinucleated giant cells. The results suggest that polysaccharide extracts obtained from tamarind pulp may contain active inhibitors of in vitro cellular multiplication.

Page(s): 169-176

DOI: DOI not available

Published: Journal: Journal of Medical Science, Volume: 5, Issue: 3, Year: 2005

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