The concentration McMaster technique is suitable for quantification of coccidia oocysts in bird droppings. | [Pakistan Veterinary Journal • 2013]

Author(s):

1. Jaroslav Vadlejch: Department of Zoology and Fisheries, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Kamýcká 957, 165 21 Prague 6-Suchdol, Czech Republic

2. Miloslav Petrtýl: Department of Zoology and Fisheries, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Kamýcká 957, 165 21 Prague 6-Suchdol, Czech Republic

3. Daniela Lukešová: Department of Animal Sciences and Food Processing in Tropics and Subtropics, Faculty of Tropical Agricultural Sciences, Czech University of Life Sciences Prague, Kamýcká 165, 165 21 Prague 6-Suchdol, Czech Republic

4. Zuzana Cadková: Department of Zoology and Fisheries, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Kamýcká 957, 165 21 Prague 6-Suchdol, Czech Republic

5. Marie Kudrnácová: Department of Zoology and Fisheries, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Kamýcká 957, 165 21 Prague 6-Suchdol, Czech Republic

6. Ivana Jankovská: Department of Zoology and Fisheries, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Kamýcká 957, 165 21 Prague 6-Suchdol, Czech Republic

7. Iva Langrová: Department of Zoology and Fisheries, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Kamýcká 957, 165 21 Prague 6-Suchdol, Czech Republic

Abstract:

The objective of this study was to evaluate the suitability of the Concentration McMaster technique in counting coccidia oocysts and to compare it with two others -McMaster method modified by Wetzel and Zajícek. A stock suspension containing Eimeria oocysts was prepared, from which five concentrations (20, 250, 500, 1,000 and 1,500) of oocysts were made. Each method was evaluated after the examination of 30 samples in five concentrations. Each sample was presented as a parasite negative fecal dropping into which a known number of oocysts were inoculated. When results of all three methods were compared, statistically significant differences (P

Page(s): 291-295

DOI: DOI not available

Published: Journal: Pakistan Veterinary Journal, Volume: 33, Issue: 3, Year: 2013

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