Abstract:
Cotton leaf curl disease, caused by begomoviruses, poses a serious threat to cotton production in Pakistan. The disease is caused by 5-6 species and numerous strains of begomoviruses. Alternate host plants are the reservoir host of economically important viruses, the source of inoculum, and the site of recombination. Leaf samples of the Hibiscus rosa-sinensis plant show vein thickening symptoms,and healthy leaf samples, with no symptoms, were collected from gardens of the National University of Sciences and Technology (NUST), Islamabad, Pakistanin 2023. PCR amplification with specific primers amplified a begomovirus and a betasatellite from symptomatic plants but not from healthy plant samples. After cloning, the genome sequence of virus was determined and shown to sharethe highest (98%) nucleotide sequence identity with Cotton leaf curl Multan virus (CLCuMV). The betasatellite showed the highest nucleotide sequence identity to an isolate of cotton leaf curl Multan betasatellite (CLCuMuB), a betasatellite associated with cotton leaf curl disease across Pakistan and northwestern India. These findings add to the diversity of known begomoviruses in South Asia and again highlight the role of hibiscus as a reservoir of the cotton leaf curl begomovirus betasatellite complex. This is the first report of cotton leaf curl Multan virus infecting Hibiscus in Pakistan.
Page(s):
130-130
DOI:
DOI not available
Published:
Journal: Abstract Book on International Conference on Food and Applied Sciences (ICFAS-23) 3-5 August 23, Volume: 0, Issue: 0, Year: 2023
Keywords:
Cotton leaf curl virus
,
Cotton
,
Cotton
,
Hibiscus
,
reservoir host