Abstract:
Plant species hybridize frequently and there are many life-history trait combinations which combine to increase the likelihood between plant species. Morphological data is required for the classification and discrimination among plant species. However, it requires comprehensive and detailed understanding of floral characteristics. To overcome this, taxonomists use molecular specific DNA markers for the identification of different species. DNA barcoding in the land plants presents a number of challenges. The objective of the current work is to study the genetic differentiation between Acacia species growing in Pattoki as plant genetic resources based on morphological (qualitative and quantitative) characters and molecular markers. The molecular identification of different taxa of genus Acacia plays a critical role to understand the evolutionary history of this important plant that has many medicinal and ecological benefits and distributed in many regions around the world. This is the first study that used DNA barcoding markers (rbcL and trnH-psbA) with 3 taxa of genus Acacia collected from Pattoki region for molecular identification, genetic resources preservation, detect genetic variations accompanied with a phylogenetic view. We describe sampling techniques, extraction protocols, and PCR methods supplementary plant DNA barcode regions. The novelty of the current study is highlighting the importance of plant genetic resources and using different techniques to measure the differentiation between species. The outcome of the present experiment was the evaluation of DNA barcoding markers for the precise identification and recognition among the prevalent Acacia species found in Pattoki, Pakistan. Recent results showed that both rbcL and trnH-psbA markers have demonstrated reasonable levels of species discrimination. It provided first report from the Pattoki region for rapid species identification of selected plant (Acacia) as well as evaluates the discrimination power of barcoding markers.
Page(s):
21-21
DOI:
DOI not available
Published:
Journal: Abstract Book on Second International Conference on Recent Approaches in Plant Sciences (RAPS-23) 4-5 May 2023 , Volume: 0, Issue: 0, Year: 2023