Agrobacterium mediated genetic transformation in lentil (Lens culinaris Medik) crop of Pakistan. | [International Journal of Biology and Biotechnology • 2012]

Author(s):

1. Muhammad Sohaib: Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi, Pakistan

2. M. Fahim Abbas: National Agricultural Research Centre, Park Road, Islamabad-45500, Pakistan

3. K. N. Shah: Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi, Pakistan

4. Saleemuddin: National Agricultural Research Centre, Park Road Islamabad-45500, Pakistan

5. S. Gull: National Agricultural Research Centre, Park Road Islamabad-45500, Pakistan

Abstract:

Lentil (Lens culinaris Medik) is ranked as sixth important pulse crop of the world. Conventional breeding in lentil is hampered due to inadequate genetic variability, high degree of self pollination and small flowers. Cotyledonary nodes (CN), epicotyls and hypocotyls of two varieties of lentil namely Masoor-85 and 2009 were used as explants source to establish a protocol for genetic transformation system using Agrobacterium tumefaciens strain (EHA105 and LBA4404) containing pGreen plasmid vector with GUS gene. Cotyledonary nodes (93%) and epicotyls (56%) of Masoor-2009 showed maximum growth on shooting media as compared with Masoor-85. Epicotyls of Masoor-2009 and Masoor-85 showed 56% and 45% regeneration with 2-3 shoots developed on each epicotyl. The performance of EHA105 and LBA4404 in GUS expression was 70% and 60% respectively. Stable integration of GUS gene was also confirmed through PCR.

Page(s): 347-351

DOI: DOI not available

Published: Journal: International Journal of Biology and Biotechnology, Volume: 9, Issue: 4, Year: 2012

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