Utilization of inulin-rich plant extracts in probiotic bacterial culture for lactase production | [Journal of Natural & Applied Sciences Pakistan • 2019]

Author(s):

1. Sarosh Khawaja: Department of Biotechnology, Kinnaird College for Women, Lahore, Pakistan

2. Sundus Mubashir: Department of Biotechnology, Kinnaird College for Women, Lahore, Pakistan

3. Shahnaz Choudhry: Department of Biotechnology, Kinnaird College for Women, Lahore, Pakistan

Abstract:

This research focuses on the isolation and identification of lactase producing probiotic bacteria in combination with inulin as a prebiotic, preparation of inulin rich garlic and onion extracts, and determination of the enzyme activity in the presence of these extracts. Streak plate method was used to obtain co-culture of Streptococcus thermophilus and Lactobacillus bulgaricus from yogurt in Streptococcus thermophilus isolation agar. After optimization of pH and temperature, lactase activity of the probiotic co-culture with 0.2%, 0.4% and 0.8% onion and garlic extracts in phenol red lactose broth was analyzed. A change in the pH of the culture from 6.6 to 3.5 was noticed at 42°C. Commercial inulin was used as positive control. Enzyme assay was performed for 48 hour culture by using Dinitrosalicyclic acid agent (DNS). Glucose standard curve was used as reference. Among three concentrations of inulin rich garlic and onion extracts used, high enzyme activity was obtained at 0.4% and 0.8%. The activity units obtained in the presence of garlic extract were 4.03 IU/ml/min and 4.29 IU/ml/min, with onion extract, the enzyme activity was 3.91 IU/ml/min and 4.03 IU/ml/min, respectively. Inulin-rich garlic extract seems to be a better choice for lactase enzyme production in the co-culture of the two probiotic strains.

Page(s): 8-17

DOI: DOI not available

Published: Journal: Journal of Natural & Applied Sciences Pakistan, Volume: 1, Issue: 1, Year: 2019

Keywords:

probiotics , probiotics , probiotics , Lactobacillus bulgaricus , inulin , Streptococcus thermophilus , Lactase enzyme

References:

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[5] . .. y it2 0.2 0.4 0.6 0, : 8.

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