Expressional Alteration of MicroRNAs in Papillary Thyroid Carcinoma | [First International Conference on Revamped Scientific Outlook of 21st Century (Abstract Book) • 2022]

Author(s):

1. Ruqia Mehmood Baig: Department of Zoology, Wildlife and Fisheries/ Biology, PMAS Arid Agricultural University,Rawalpindi,Pakistan

2. Nafeesa Kainat: Department of Zoology, Wildlife and Fisheries/ Biology, PMAS Arid Agricultural University,Rawalpindi,,Pakistan

3. Afshan Afzal: Department of Zoology, Wildlife and Fisheries/ Biology, PMAS Arid Agricultural University,Rawalpindi,,Pakistan

4. Rashida Khan: Department of Zoology, Wildlife and Fisheries/ Biology, PMAS Arid Agricultural University,Rawalpindi,,Pakistan

Abstract:

Papillary Thyroid Cancer (PTC) is a most prevalent type of thyroid cancer accounts for over 80% of all thyroid cancer cases. In last two decades, prevalence of PTC has increased worldwide as well as in Pakistan. Innovative molecular diagnostic and therapeutic options are suggested for diagnostic and treatments of thyroid cancer. microRNAs or miRNAs are endogenous, single-stranded non-coding RNAs that are around 20-22nucleotides long. microRNAs greatly influence the control of several vital functions, including hematopoiesis, immunological responses, proliferation of cells, and programmed cell death. miRNAs have a significant role in the initiation, development, and progression of a variety of human cancers. MicroRNAs and cancer have a strong correlation, and papillary thyroid carcinoma has been linked to deregulation of numerous microRNAs. Aim of this study was to evaluate the miRNA expression pattern in PTC using Real time PCR. Two microRNAs mir-21 and mir-31were selected for present study. Confirmed PTC patients (n=50) from hospitals of Rawalpindi and Islamabad were recruited in the present study. Tissue samples and adjacent healthy samples were collected in RNA later followed by RNA extraction by TRIzol method and amplification with specific primers by Real time PCR. Fold change method was used to find out expression level of miRNAs in PTC. Student's t-test was used to evaluate the difference in expression of respective miRNAs in disease and healthy samples. P < 0.05 was taken as significant and statistical analyses were carried out by using SPSS software. This study showed that level of mir-21 was up regulated (fold change 1 to 12) in PTC tissues and was positively correlated with older age and high TSH level. While mir-31expression was observed to be significantly down regulated (3.7 to -85.2) in PTC tissues as3compare to normal adjacent healthy sample (P<0.05). Expression analysis of studied miRNAs has increased the possibility for using miRNAs as cancer biomarkers and therapeutic targets. Regression analyses of miRNAs expression and various hormones suggested that high TSH level might play an important functional role in PTC tumorigenesis. Both of the studied micro RNAs were observed in strong association with thyroid cancer. It is predicted through this study that these miRNAs can be used as diagnostic and prognostic markers in the early detection of thyroid cancer in Pakistani population.

Page(s): 0-0

DOI: DOI not available

Published: Journal: First International Conference on Revamped Scientific Outlook of 21st Century (Abstract Book), Volume: 0, Issue: 0, Year: 2022

Keywords:

Thyroid cancer , thyroid cancer , MicroRNA , Papillary Thyroid cancer , Expressional analysis

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