Author(s):
1. Aimen Chaudhry:
Department of Biochemistry, IBBB, The Islamia University of Bahawalpur, Bahawalpur, Pakistan
Abstract:
This study focuses on screening, extraction and characterization of pathologically important enzyme lipase from Aspergillus niger isolated from patients of Bahawalpur region capable of producing lipolytic enzymes. The only pathogenic side of the fungus involve skin or scalp infections and rarely lungs infections in immuno-compromised persons. The study involved the morphological characterization of the pathologically important lipase enzyme extracted from A. niger which involves the effect of different temperature, pH, enzyme loading and different oils on enzyme activity. The varying effect of pH and temperature shows that the potential lipase was most active at pH 7 and at 450C. The variable concentration of enzyme loading with 20(w/v) of weight of oil indicates high lipase production and proving physic nut oil as the best inducer of lipase production from A. niger. This study determined important activity factors of lipase enzyme extracted from Bahawalpur, Pakistan. The research work used simplest and economical ways of extracting lipase enzyme such as Solid State Fermentation having a solid substrate bought from a local market. Finally, it can be concluded that the lipase enzyme which is extracted from Bahawalpur region based strain of A.niger is a pathologically important enzyme and can show its specific activity under predetermined characterization parameters.
Page(s):
94-94
DOI:
DOI not available
Published:
Journal: Abstract Book on Global Science Technology and Management Conference, Volume: 0, Issue: 0, Year: 2023
Keywords:
Morphological characterization
,
Aspergillus species
,
pathological important enzyme
References:
References are not available for this document.
Citations
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