Abstract:
Ascorbic acid is an important bio molecule for human bodies. When the level of Ascorbic acid in human body is abnormal then they causes various diseases such as high blood pressure, hypertension, heart diseases, high cholesterol, gout and kidney diseases. In the present study we designed Nickel doped Hydroxyapatite based biosensor for the detection of Ascorbic acid in gouts patient's blood serum sample. The characterization of synthesized Nickel doped Hydroxyapatite nanocomposite was done by various techniques such as FTIR, SEM and XRD. The ionic Nickel doped Hydroxyapatite nanocomposite shows intrinsic peroxidase-like activity, and in the presence of H2O2 the oxidation of the peroxidase substrate 3,3',5,5'- tetramethylbenzidine could be catalyze into a greenish colored product. Based on this phenomenon, for the colorimetric determination of ascorbic acid in human serum sample Nickel doped Hydroxyapatite nanocomposite based sensor was developed. The optimization of the proposed sensor was done with different parameters such as (amount of capping agent, H2O2, pH, TMB, time and uric acid concentration) to get the best results. The proposed sensor provide a sensitive and simple effect for uric acid detection using TMB, H2O2 and Nickel doped Hydroxyapatite nanocomposite with a linear range from 2 x 10-9-3.20 x 10-7 M with low limit of detection 1.7x10-8 M, low limit of quantification 5.6x10-8 M and R20.9994. For the colorimetric detection of ascorbic acid the proposed sensor gave a short response time at 4 min, room temperature and pH 7.5. Moreover, for the detection of ascorbic acid in human serum samples the proposed method can be successfully applied.
Page(s):
433-433
DOI:
DOI not available
Published:
Journal: Abstract Book on International Conference on Food and Applied Sciences (ICFAS-23) 3-5 August 23, Volume: 0, Issue: 0, Year: 2023
Keywords:
Hydrogen peroxide
,
ascorbic acid
,
Colorimetric sensor
,
Nickel doped Hydroxyapatite nanocomposite
,
peroxidase mimic