Abstract:
In this study, the contents of nitrite-nitrate and free L-proline, and pathogenesis-related (PR) proteins in tomato plants following inoculation with Pseudomonas syringae pv tomato strain were examined The results of the nitrite and nitrate indicated that there was a reduction in the levels of nitrate in the infected tomato plants through 1-8 study days, compared with the healthy plants On the other hands, when the nitrite amounts increased in the first and second days, the nitrite concentrations reduced in infected plants at subsequent time periods, compared with uninfected plants The accumulation of free proline increased in the infected plants, according to control plants The whole-cell protein profiles displayed that the levels of the protein bands of molecular masses 2046 kDa and 699 kDa significantly increased in infected and uninfected plants during 2-10 study days In additionally, in the quantities of the protein bands of molecular weights 903 and 794 kDa were observed an increase in the infected and healthy plants after the fourth day However, the protein band of molecular weight 543 kDa was visible only in uninfected plants for the fourth and eighth days Finally, the study suggest that there were the sophisticate relationships among the proline accumulation, the conversion of nitrate to nitrite and the induction of PR protein genes in the regulation of defense mechanisms toward microbial invaders Our results also indicated that the increases in nitrite and proline contents might be useful indicator for the response toward pathogen attacks.
Page(s):
1521-1526
DOI:
DOI not available
Published:
Journal: Pakistan Journal of Botany, Volume: 44, Issue: 5, Year: 2012