Effect of Dilute Sulphuric Acid Pretreatment on Cellulase Production by Bacillus subtilis (K-18) through Response Surface Methodology | [Proceedings of the Pakistan Academy of Sciences: B. Life and Environmental Sciences • 2017]

Author(s):

1. Anam Arooj: Microbial Biotechnology Laboratory, Department of the Zoology, University of the Punjab,Lahore,Pakistan

2. Muhammad Irfan: Department of Biotechnology, University of Sargodha,Sargodha,Pakistan

3. Fouzia Tabbasum: Department of Biotechnology, University of Sargodha,Sargodha,Pakistan

4. Hafiz Abdullah Shakir: Microbial Biotechnology Laboratory, Department of the Zoology, University of the Punjab,Lahore,Pakistan

5. Javed Iqbal Qazi: Microbial Biotechnology Laboratory, Department of the Zoology, University of the Punjab,Lahore,Pakistan

Abstract:

The present study investigated the optimization of dilute sulphuric acid pretreatment to maximize cellulase production from banana peduncle waste through Box-Behnken design of response surface methodology. Cellulase production was carried out in 250ml capacity Erlenmeyer flask using pretreated banana peduncle as substrate in submerged fermentation by Bacillus subtilis K-18 incubated at 50oC for fermentation period of 24 h. Results indicated that chemical pretreatment using sulphuric acid favored cellulase production as compared to thermochemical pretreatment using sulphuric acid followed by autoclaving at 121oC for 15 min and 15 psi. Maximum Filter Paper activity of 0.958 IU/ml/min was observed at optimal pretreatment conditions of 0.4 N H2SO4 concentration, 15% substrate concentration and residence time of 6h with chemical pretreatment. For thermochemical pretreatment optimal FPase activity of 0.63 IU/ml/min was recorded at 0.4 N H2SO4 concentration, 10% substrate concentration and residence time of 4 h. The proposed regression model for both types of pretreatments was found significant as revealed by F-value, P-value and coefficient of determination. These results indicated that banana peduncle can be successfully utilized as solid substrate in submerged fermentation for cellulase enzyme production.

Page(s): 11-20

DOI: DOI not available

Published: Journal: Proceedings of the Pakistan Academy of Sciences: B. Life and Environmental Sciences, Volume: 54, Issue: 1, Year: 2017

Keywords:

cellulase , Submerged fermentation , Bacillus subtilis , RSM , Pretreatment

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