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Variation in Rhizobium and Azospirillum strains isolated from maize growing in arid and semiarid areas.
Author(s):
1. Noshin Ilyas: Department of Plant Sciences, Quaid-e-Azam University, Islamabad, Pakistan
2. Asghari Bano: Department of Plant Sciences, Quaid-e-Azam University, Islamabad, Pakistan
3. Sumera Iqbal: Department of Plant Sciences, Quaid-e-Azam University, Islamabad, Pakistan
Abstract:
Plant growth promoting rhizobacteria (Rhizobium & Azospirillum) were isolated from the roots and rhizosphere of maize plants grown at farmer’s field of arid and semiarid areas and irrigated area. Rhizobium and Azospirillum isolated from irrigated areas showed higher colony count as compared to those from semi-arid areas particularly at reproductive stage. For Rhizobium, sodium citrate, acid from maltose, tryptophan deaminase, indole and acid from melibiose tests were negative for isolates from low soil moisture. The results for sodium citrate, acid from arabinose and lysine decarboxylase were negative for Azospirillum from low soil moisture. On the basis of carbon/nitrogen utilization pattern, three groups of Rhizobium isolates were identified that fell into two groups on the basis of banding pattern of RAPD. Similar grouping were recognized for Azospirillum on the basis of carbon/nitrogen utilization pattern and RAPD analysis.
Page(s): 612-618
DOI: DOI not available
Published: Journal: International Journal of Agriculture and Biology, Volume: 10, Issue: 6, Year: 2008
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